Application of LS 7500 counter (Beckman) for measurement of phagocytosis of 14C labeled bacteria.

For determination of phagocytosis of Staphylococcus aureus 519 labeled with glycine-1-14C by guinea pig granulocytes, the third analytical program of LS 7500 counter was used. By measuring isotope radioactivity, the program enables evaluation of the percentage of a bound isotope in relation to the reference sample. In the present experiments the percentage of a bound isotope stands for the percentage of phagocytized bacteria calculated in relation to a reference sample, i.e. to radioactivity of bacteria added to the test. The program applied allows an automatic calculation of the percentage of phagocytized bacteria, the result being printed by a counter printer.