Lu Lu, Chen Xinying, Zhu Liming, Li Mengjuan, Zhang Jingbo, Yang Xiuyan, Wang Pengkai, Lu Ye, Cheng Tielong, Shi Jisen, Yi Yin, Chen Jinhui
Key Laboratory of Forestry Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.
Experimental Center of Desert Forestry, Chinese Academy of Forestry, Dengkou, China.
Front Plant Sci. 2020 Aug 21;11:1112. doi: 10.3389/fpls.2020.01112. eCollection 2020.
Calcineurin B-like protein-interacting protein kinases () play essential roles in plant abiotic stress response. In order to better understand salt tolerance, we cloned and analyzed the gene from the halophyte . Phylogenetic analysis shows that belongs to a sister clade with the gene and is thought to localize to the plasma membrane. shows the highest expression level in the root under normal growth conditions, whereas after NaCl treatment, the highest expression was found in the blade. -overexpressing Arabidopsis plants have a higher seed germination rate, longer root length, and displayed higher salt tolerance than wild type seedlings under salt stress conditions. Furthermore, overexpression might enhance the expression of genes related to K transportation after NaCl treatment. Thus, we conclude that increases transgenic plant salt tolerance and reduces damage associated with salt stress by promoting the expression of genes controlling ion homeostasis. Our results suggest that could serve as an ideal candidate gene to genetically engineer salt-tolerant plants.
类钙调神经磷酸酶B互作蛋白激酶(CIPKs)在植物非生物胁迫响应中发挥着重要作用。为了更好地理解耐盐性,我们从盐生植物中克隆并分析了基因。系统发育分析表明,与基因属于姐妹进化枝,并且被认为定位于质膜。在正常生长条件下,在根中表达水平最高,而在NaCl处理后,在叶片中发现最高表达。过表达的拟南芥植物具有更高的种子萌发率、更长的根长,并且在盐胁迫条件下比野生型幼苗表现出更高的耐盐性。此外,过表达可能会增强NaCl处理后与钾运输相关基因的表达。因此,我们得出结论,通过促进控制离子稳态的基因表达,提高了转基因植物的耐盐性并减少了与盐胁迫相关的损伤。我们的结果表明,可作为遗传工程培育耐盐植物的理想候选基因。