Lindbäck B, Berlin T, Björkhem I
Clin Chem. 1987 Jul;33(7):1226-7.
The accuracy of three commercial kit methods and one liquid-chromatographic (HPLC) method for determining 25-hydroxyvitamin D3 in serum was evaluated by using isotope dilution-mass spectrometry as a reference technique. The kit methods were based on radioimmunoassay and radioreceptor assay. Serum samples were analyzed from male and female volunteers, some of whom had been exposed to ultraviolet B light or given vitamin D3 orally. Results obtained with the three commercial kits were less accurate than those by HPLC. The agreement between the HPLC method and the comparison method was relatively good: r = 0.99; slope = 1.23; intercept = -2.9 micrograms/L. We conclude that methods based on HPLC should be preferred in routine clinical work and that the accuracy of the three commercial kits tested is not sufficient for their intended use.
通过使用同位素稀释质谱法作为参考技术，评估了三种商用试剂盒方法和一种液相色谱（HPLC）方法测定血清中25-羟基维生素D3的准确性。试剂盒方法基于放射免疫分析和放射受体分析。对男性和女性志愿者的血清样本进行了分析，其中一些人曾暴露于紫外线B光下或口服维生素D3。三种商用试剂盒获得的结果不如HPLC法准确。HPLC法与比较法之间的一致性相对较好：r = 0.99；斜率 = 1.23；截距 = -2.9微克/升。我们得出结论，在常规临床工作中应首选基于HPLC的方法，并且所测试的三种商用试剂盒的准确性不足以满足其预期用途。
