Institute of Milk Hygiene, Milk Technology and Food Science, Department of Veterinary Public Health and Food Science, University of Veterinary Medicine, Vienna, Vienna, Austria.
Austrian Agency for Health and Food Safety (AGES), Vienna, Austria.
Methods Mol Biol. 2021;2220:89-103. doi: 10.1007/978-1-0716-0982-8_7.
Nucleotide sequence-based methods focusing on the single-nucleotide polymorphisms (SNPs) of Listeria monocytogenes and L. innocua housekeeping genes (multilocus sequence typing) and in the core genome (core genome MLST) facilitate the rapid and interlaboratory comparison in open accessible databases as provided by Institute Pasteur ( https://bigsdb.web.pasteur.fr/listeria/listeria.html ). Strains can be compared on a global level and help to track forward and trace backward pathogen contamination events in food processing facilities and in outbreak scenarios.
基于核苷酸序列的方法主要关注李斯特菌和无害李斯特菌的单核苷酸多态性（SNP），以及在核心基因组（核心基因组 MLST）中的管家基因（多位点序列分型），这使得在巴斯德研究所（https://bigsdb.web.pasteur.fr/listeria/listeria.html）提供的开放可访问数据库中进行快速和实验室间的比较成为可能。可以在全球范围内对菌株进行比较，有助于追踪食品加工设施和疫情爆发场景中的病原体污染事件。
