Matsuhashi S, Watanabe T, Hori K
Exp Cell Res. 1987 Jun;170(2):351-62. doi: 10.1016/0014-4827(87)90312-0.
A monoclonal antibody Pr-28 was prepared, which recognized an antigen present only in proliferating cells. Immunofluorescence analysis of Pr-28 antigen showed that the antigen was localized mainly in perinuclear cytoplasm. Although Pr-28 antibody was produced against a chicken cell antigen, it reacts not only with chicken cells but also other cells of murine origin, such as L-cells and NIH 3T3 cells. The molecular weight (Mr) of the antigen recognized by Pr-28 antibody was 45,000 D as determined by SDS-PAGE run under reducing conditions. The antigen disappeared in NIH 3T3 quiescent cells, reappearing in quiescent cells stimulated by fetal calf serum (FCS). The synthesis of Mr 45,000 protein occurred at late G1 phase, just before DNA synthesis in serum-stimulated quiescent NIH 3T3 cells and ceased in S phase.
制备了一种单克隆抗体Pr - 28，它识别一种仅存在于增殖细胞中的抗原。对Pr - 28抗原的免疫荧光分析表明，该抗原主要定位于核周细胞质中。尽管Pr - 28抗体是针对鸡细胞抗原产生的，但它不仅与鸡细胞反应，还与鼠源的其他细胞反应，如L细胞和NIH 3T3细胞。在还原条件下进行的SDS - PAGE分析确定，Pr - 28抗体识别的抗原分子量（Mr）为45,000 D。该抗原在NIH 3T3静止细胞中消失，在胎牛血清（FCS）刺激的静止细胞中重新出现。在血清刺激的静止NIH 3T3细胞中，Mr 45,000蛋白的合成发生在G1期晚期，就在DNA合成之前，并在S期停止。
