Department of Blood Transfusion, Sir Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China.
Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou 310029, China.
J Glob Antimicrob Resist. 2020 Dec;23:181-183. doi: 10.1016/j.jgar.2020.09.007. Epub 2020 Sep 23.
Salmonella enterica serovar Goldcoast has been identified as the agent responsible for multistate epidemic outbreaks in humans. However, knowledge regarding the antimicrobial resistance and transmission of S. Goldcoast is scarce. This study aimed to investigate the genomic features of a multidrug-resistant S. Goldcoast sequence type 358 strain in China.
Antimicrobial susceptibility of S. enterica strain SalGC_ZJ_53 was determined by microdilution broth assay. Whole genomic DNA was extracted and sequenced using the Illumina NovaSeq platform. De novo genome assembly was performed using Unicycler and the draft genome was annotated using the NCBI Prokaryotic Genome Annotation Pipeline. The sequence type (ST), antimicrobial resistance genes and Salmonella pathogenicity islands were identified from the genome sequence. The core genome multilocus sequence typing (cgMLST) analysis between S. enterica SalGC_ZJ_53 and all of the S. Goldcoast strains retrieved from the public database was performed using BacWGSTdb server.
Salmonella enterica SalGC_ZJ_53 was resistant to ampicillin, amikacin, cefotetan, cefazolin, ciprofloxacin, gentamicin and tetracycline. The genome size was calculated as 4,904,788 bp, with 4595 protein-coding sequences and GC content of 52.0%. This isolate was assigned to ST358. Several antimicrobial resistance genes and salmonella pathogenicity islands, as well as multiple insertion sequences were identified. The closest relative of S. enterica SalGC_ZJ_53 was another isolate recovered from the UK, differing by only six cgMLST loci.
This study reports the first genome sequence of a multidrug-resistant S. Goldcoast isolate in China. These data may help to understand the antimicrobial resistance mechanisms and transmission dynamics of this rare serovar of Salmonella infection in humans.
肠沙门氏菌血清型 Goldcoast 已被确定为导致人类多州疫情爆发的病原体。然而，有关 S. Goldcoast 的抗生素耐药性和传播的知识却很少。本研究旨在研究中国一株多药耐药性 S. Goldcoast 序列型 358 菌株的基因组特征。
采用微量稀释肉汤法测定肠沙门氏菌菌株 SalGC_ZJ_53 的药敏性。提取全基因组 DNA，采用 Illumina NovaSeq 平台进行测序。使用 Unicycler 进行从头基因组组装，使用 NCBI Prokaryotic Genome Annotation Pipeline 对草图基因组进行注释。从基因组序列中鉴定序列型（ST）、抗生素耐药基因和沙门氏菌致病岛。使用 BacWGSTdb 服务器对 S. enterica SalGC_ZJ_53 与从公共数据库中检索到的所有 S. Goldcoast 菌株的核心基因组多位点序列分型（cgMLST）分析。
肠沙门氏菌 SalGC_ZJ_53 对氨苄西林、阿米卡星、头孢替坦、头孢唑林、环丙沙星、庆大霉素和四环素耐药。基因组大小计算为 4904788bp，有 4595 个蛋白编码序列，GC 含量为 52.0%。该分离株被分配到 ST358。鉴定出多个抗生素耐药基因和沙门氏菌致病岛，以及多个插入序列。与 S. enterica SalGC_ZJ_53 最接近的亲缘关系是从英国分离的另一个分离株，仅在 6 个 cgMLST 基因座上存在差异。
本研究报告了中国首例多药耐药性 S. Goldcoast 分离株的全基因组序列。这些数据可能有助于了解这种人类沙门氏菌感染罕见血清型的抗生素耐药机制和传播动态。
