两株从埃及零售鸡肉中分离的耐环丙沙星肠炎沙门氏菌血清型肯塔基 ST198 的基因组草图序列。
Draft genome sequences of two ciprofloxacin-resistant Salmonella enterica subsp. enterica serotype Kentucky ST198 isolated from retail chicken carcasses in Egypt.
机构信息
Bacterial Epidemiology and Antimicrobial Resistance Research Unit, US National Poultry Research Center, USDA-ARS, Athens, GA 30605, USA; Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Bacterial Epidemiology and Antimicrobial Resistance Research Unit, US National Poultry Research Center, USDA-ARS, Athens, GA 30605, USA.
出版信息
J Glob Antimicrob Resist. 2018 Sep;14:101-103. doi: 10.1016/j.jgar.2018.06.012. Epub 2018 Jun 30.
OBJECTIVES
Salmonella enterica serotypes, particularly antimicrobial-resistant strains, pose a major threat to public health worldwide. This study describes the draft genome sequences of two ciprofloxacin-resistant Salmonella enterica subsp. enterica serotype Kentucky isolates (H5 and H18) recovered from chicken carcass rinsates in Mansoura, Egypt.
METHODS
Antimicrobial susceptibility phenotypes were determined for the two Salmonella Kentucky isolates by broth microdilution using a Sensititre™ system. Genomic DNA from both isolates was sequenced using an Illumina MiSeq system. Antimicrobial resistance genes were identified using ARG-ANNOT, and multilocus sequence typing (MLST) was performed using MLST 1.8.
RESULTS
The draft genome for Salmonella Kentucky H5 contained 4.84Mbp in 54 contigs, and that for Salmonella Kentucky isolate H18 contained 4.94Mbp in 64 contigs. Sequence analysis using ARG-ANNOT identified the presence of the resistance genes bla, aadA1, aadA2, cmlA1, sul3 and tetA in both isolates, whereas dfrA, sul2, floR, and aph(3)-Ia were found in isolate H18 only. The amino acid substitutions Ser83Phe and Asp87Gly in GyrA and Thr57Ser and Ser80Ile in ParC were detected in both isolates. Both isolates belonged to ST198.
CONCLUSION
The draft genome sequences allowed identification of a ciprofloxacin-resistant Salmonella Kentucky ST198 epidemic clone with multidrug resistance in poultry products produced for human consumption in Egypt. These data indicate that poultry continues to be a reservoir for this persistent clone.
目的
肠沙门氏菌血清型，尤其是具有抗药性的菌株，对全球公共健康构成了重大威胁。本研究描述了从埃及曼苏拉市鸡胴体冲洗液中分离出的两株环丙沙星耐药肠沙门氏菌亚种肠炎血清型肯塔基州分离株（H5 和 H18）的草图基因组序列。
方法
采用 Sensititre™系统通过肉汤微量稀释法测定两株沙门氏菌肯塔基州分离株的药敏表型。使用 Illumina MiSeq 系统对两株分离株的基因组 DNA 进行测序。使用 ARG-ANNOT 鉴定抗生素耐药基因，使用 MLST 1.8 进行多位点序列分型（MLST）。
结果
沙门氏菌肯塔基州 H5 的草图基因组包含 4.84Mbp 的 54 个连续序列，而沙门氏菌肯塔基州 H18 的草图基因组包含 4.94Mbp 的 64 个连续序列。使用 ARG-ANNOT 进行序列分析鉴定出两株菌均存在 bla、aadA1、aadA2、cmlA1、sul3 和 tetA 耐药基因，而 dfrA、sul2、floR 和 aph(3)-Ia 仅在 H18 中发现。在两株菌中均检测到 GyrA 中的 Ser83Phe 和 Asp87Gly 以及 ParC 中的 Thr57Ser 和 Ser80Ile 氨基酸取代。两株菌均属于 ST198。
结论
草图基因组序列鉴定出了在埃及用于人类消费的禽产品中具有多药耐药性的环丙沙星耐药沙门氏菌肯塔基州 ST198 流行克隆。这些数据表明，禽类仍然是该持久性克隆的储存库。
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