Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; Key Laboratory of Crop and Livestock Integrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210095, China.
Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China.
Bioresour Technol. 2021 Jan;319:124149. doi: 10.1016/j.biortech.2020.124149. Epub 2020 Sep 21.
This study investigated the effect of thermal pretreatment (TP) on the lignocellulose degradation and humification during dairy manure composting and the underlying microbial mechanism. The results showed that TP accelerated temperature rise and elevated composting temperature by increasing 26% initial content of simple organics. The degradation of cellulose, hemicellulose and lignin was 78, 10 and 109% higher in thermal pretreatment composting (TPC) than traditional composting (TC), respectively. Moreover, TP significantly improved the humification degree of composts, as indicated by 14 and 38% higher humus content and humification indexes in TPC, respectively. 16S rRNA sequencing showed that TP increased the relative abundance of thermophilic bacteria in TPC, of which Thermobifida, Planifilum, Truepera and Thermomonospora were potentially involved in lignocellulose biodegradation and humification. Canonical correspondence analysis revealed that TP changed the main factor determining the bacterial community evolution from dissolved organic carbon (DOC) in TC to temperature in TPC.
本研究考察了热预处理（TP）对奶牛粪便堆肥过程中木质纤维素降解和腐殖化的影响及其潜在的微生物机制。结果表明，TP 通过增加 26%初始简单有机物含量来加速升温并提高堆肥温度。与传统堆肥（TC）相比，热预处理堆肥（TPC）中纤维素、半纤维素和木质素的降解率分别提高了 78%、10%和 109%。此外，TP 显著提高了堆肥的腐殖化程度，TPC 中的腐殖质含量和腐殖化指数分别提高了 14%和 38%。16S rRNA 测序表明，TP 增加了 TPC 中嗜热细菌的相对丰度，其中 Thermobifida、Planifilum、Truepera 和 Thermomonospora 可能参与了木质纤维素的生物降解和腐殖化。典范对应分析表明，TP 改变了决定细菌群落演替的主要因素，从 TC 中的溶解有机碳（DOC）变为 TPC 中的温度。
