School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
Waste Manag. 2021 May 15;127:1-9. doi: 10.1016/j.wasman.2021.04.033. Epub 2021 Apr 25.
The purpose of this study was to analyze the effects of illite/smectite clay (I/S) on lignocellulosic degradation and humification process via metagenomics analysis during cattle manure composting. The test group (TG) with 10% I/S and the reference group (RG) were established. The results indicated that the addition of I/S made the degradation rate of cellulose, hemicellulose and lignin in TG (1.56%, 29.01%, 19.95%) was higher than that in RG (1.16%, 17.24%, 13.14%). Compared with RG, the abundance values of AA2, AA10, GH1 and GH10 in TG increased by 15.18%, 29.28%, 31.08%, 21.65%, respectively. Meanwhile, humic substance (HS) content was increased by 3.49% and 7.16% during RG and TG composting. Furthermore, the microbial community in TG changed, in which the relative abundance of Actinobacteria increased and Proteobacteria decreased. Redundancy analysis (RDA) showed that the temperature was positively correlated with the abundance of AA2, AA10, GH1 and GH10, whereas the organic matter content was negatively correlated. Overall, adding I/S to the composting could stimulate microbial activity, promote the degradation of lignocellulose and humification process.
本研究旨在通过宏基因组分析，分析伊利石/蒙脱石粘土（I/S）对牛粪堆肥过程中木质纤维素降解和腐殖化过程的影响。设置添加 10%I/S 的实验组（TG）和对照组（RG）。结果表明，I/S 的添加使 TG 中纤维素、半纤维素和木质素的降解率（1.56%、29.01%、19.95%）高于 RG（1.16%、17.24%、13.14%）。与 RG 相比，TG 中 AA2、AA10、GH1 和 GH10 的丰度值分别增加了 15.18%、29.28%、31.08%和 21.65%。同时，RG 和 TG 堆肥过程中腐殖质（HS）含量分别增加了 3.49%和 7.16%。此外，TG 中的微生物群落发生了变化，其中放线菌的相对丰度增加，而变形菌的相对丰度减少。冗余分析（RDA）表明，温度与 AA2、AA10、GH1 和 GH10 的丰度呈正相关，而与有机质含量呈负相关。总体而言，向堆肥中添加 I/S 可以刺激微生物活性，促进木质纤维素的降解和腐殖化过程。
