多孔基质与功能菌群协同作用在低温下提高两阶段潮汐流人工湿地的养分去除效率：中试规模研究。
The synergy of porous substrates and functional genera for efficient nutrients removal at low temperature in a pilot-scale two-stage tidal flow constructed wetland.
机构信息
College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China.
College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China; China Architecture Design and Research Group, Beijing 100044, China.
出版信息
Bioresour Technol. 2021 Jan;319:124135. doi: 10.1016/j.biortech.2020.124135. Epub 2020 Sep 17.
A pilot-scale two-stage tidal flow constructed wetland (TFCW) with working volume of 0.46 m/d packing with shale ceramsite (SC) and activated alumina (AA) was constructed (named as SC-AA-TFCW) for nutrients removal at low temperature (<15 °C). SC-AA-TFCW achieved stable removals of 78.1% nitrogen and 98.3% phosphorous. SC-TFCW contributed to 55.2% of organics and 85.6% of particulate phosphorous removal. Among 17 denitrifiers, the absolute abundance of aerobic denitrification bacteria (ADNB) was highest, followed by facultative anaerobic denitrification bacteria (FADNB) and autotrophic denitrification bacteria (AUDNB). Nitrogen assimilating into organic nitrogen, dissimilatory and assimilatory nitrate reduction and complete denitrification may be main nitrogen metabolic pathways. Some ADNB (e. g. Zoogloea, Pseudomonas and Acidovorax) showed positive interactions with various key functional genes related to nutrients removal. Dissolved oxygen and reducing elements were main environmental factors in changing ADNB compositions. This study highlights the importance of ADNB and their synergy to porous substrates in SC-AA-TFCW.
采用工作体积为 0.46 m/d 的两级潮汐流人工湿地（TFCW），填充页岩陶粒（SC）和活性氧化铝（AA），构建了用于低温（<15°C）下脱除营养物的中试规模 SC-AA-TFCW。SC-AA-TFCW 实现了 78.1%的氮去除率和 98.3%的磷去除率。SC-TFCW 对有机物的去除率为 55.2%，对颗粒态磷的去除率为 85.6%。在 17 种反硝化菌中，好氧反硝化菌（ADNB）的绝对丰度最高，其次是兼性厌氧反硝化菌（FADNB）和自养反硝化菌（AUDNB）。氮同化进入有机氮、异化和同化硝酸盐还原以及完全反硝化可能是主要的氮代谢途径。一些 ADNB（如动胶菌属、假单胞菌属和食酸菌属）与各种与营养物去除相关的关键功能基因表现出正相互作用。溶解氧和还原元素是改变 ADNB 组成的主要环境因素。本研究强调了 ADNB 及其与 SC-AA-TFCW 多孔基质协同作用的重要性。
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