Neural Developmental Biology Lab, Department of Life Science, NIT Rourkela, Rourkela, Odisha 769008, India.
Division of Pharmaceutical and Fine Chemical Technology, Department of Chemical Technology, University of Calcutta, West Bengal 700009, India.
Int J Biol Macromol. 2020 Dec 15;165(Pt A):333-345. doi: 10.1016/j.ijbiomac.2020.09.144. Epub 2020 Sep 25.
The current study aims to check various behavioural, developmental, cytotoxic, and genotoxic effects of FeO-GG nanocomposite (GGNCs) on Drosophila melanogaster. FeO nanoparticles were prepared by the chemical co-precipitation method and cross-linked with guargum nanoparticles to prepare the nanocomposites. The nanocomposites were characterized by using transmission electron microscopy (TEM), X-ray diffraction (XRD), and FTIR techniques. To investigate the biomolecular interaction, GGNCs was further tagged with Fluorescein isothiocyanate. Various concentrations of nanocomposites were mixed with the food and flies were allowed to complete the life cycle. The life cycle of the flies was studied as a function of various concentrations of GGNCs. The 1st instar larvae after hatching from the egg start eating the food mixed with GGNCs. The 3rd instar larvae were investigated for various behavioural and morphological abnormalities within the gut. The 3rd instar larva has defective crawling speed, crawling path, and more number of micronuclei within the gut. Similarly, in adult flies thermal sensitivity, climbing behaviour was found to be altered. In adult flies, a significant reduction in body weight was found which is further correlated with variation of protein, carbohydrate, triglyceride, and antioxidant enzymes. Altogether, the current study suggests GGNCs as a non-genotoxic nanoparticle for various biological applications.
本研究旨在检测 FeO-GG 纳米复合材料（GGNCs）对黑腹果蝇的各种行为、发育、细胞毒性和遗传毒性影响。FeO 纳米粒子通过化学共沉淀法制备，并与瓜尔胶纳米粒子交联制备纳米复合材料。利用透射电子显微镜（TEM）、X 射线衍射（XRD）和傅里叶变换红外（FTIR）技术对纳米复合材料进行了表征。为了研究生物分子相互作用，GGNCs 进一步与异硫氰酸荧光素标记。将不同浓度的纳米复合材料与食物混合，让苍蝇完成生命周期。研究了苍蝇的生命周期与 GGNCs 不同浓度的关系。从卵中孵化出的 1 龄幼虫开始食用混合有 GGNCs 的食物。研究了 3 龄幼虫在肠道内的各种行为和形态异常。3 龄幼虫爬行速度、爬行路径受损，肠道内有更多的微核。同样，在成年苍蝇中，发现热敏感性、攀爬行为发生改变。在成年苍蝇中，体重显著下降，这与蛋白质、碳水化合物、甘油三酯和抗氧化酶的变化进一步相关。总的来说，本研究表明 GGNCs 是一种非遗传毒性的纳米粒子，可用于各种生物应用。
