Biophotonics Lab, Department of Bioengineering, Birla Institute of Technology-Mesra, Ranchi, JH 835215, India.
Biophotonics Lab, Department of Bioengineering, Birla Institute of Technology-Mesra, Ranchi, JH 835215, India.
Photodiagnosis Photodyn Ther. 2021 Mar;33:102027. doi: 10.1016/j.pdpdt.2020.102027. Epub 2020 Sep 24.
We demonstrate a photothermal optical coherence tomography (PTOCT) system, with upconversion nanoparticles (UCNPs) as a molecular probe. We synthesized hydrophilic, biocompatible upconversion nanoparticles (UCNPs) using hydrothermal synthesis. We developed the PTOCT system along with the signal processing tool and applied this technique on animal tissue phantom for targeted imaging. The 'lock-in detection' of the amplitude modulated photothermal beam (980 nm), which used to excite the UCNPs was the backbone of the signal processing algorithm. The signal processing was further established in different aspects. As an application part, the diffusion dynamics of the UCNPs was performed inside the tissue to study molecular movement and subsequent changes in tissue properties. A comparison of photothermal optical coherence tomography (PTOCT) with phase variance optical coherence tomography (PVOCT) for targeted molecular imaging also presented.
我们展示了一种基于上转换纳米粒子（UCNPs）作为分子探针的光热光相干断层扫描（PTOCT）系统。我们使用水热合成法合成了亲水性、生物相容性的上转换纳米粒子（UCNPs）。我们开发了 PTOCT 系统以及信号处理工具，并将该技术应用于动物组织体模进行靶向成像。用于激发 UCNPs 的振幅调制光热光束（980nm）的“锁相检测”是信号处理算法的核心。该信号处理在不同方面得到了进一步的建立。作为应用部分，在组织内部进行 UCNPs 的扩散动力学研究，以研究分子运动和组织性质的后续变化。还提出了用于靶向分子成像的光热光相干断层扫描（PTOCT）与相位方差光相干断层扫描（PVOCT）的比较。
