Tucker-Schwartz Jason M, Beavers Kelsey R, Sit Wesley W, Shah Amy T, Duvall Craig L, Skala Melissa C
Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37235, USA.
Interdisciplinary Graduate Program in Materials Science, Vanderbilt University, Nashville, TN 37235, USA.
Biomed Opt Express. 2014 May 1;5(6):1731-43. doi: 10.1364/BOE.5.001731. eCollection 2014 Jun 1.
Current imaging techniques capable of tracking nanoparticles in vivo supply either a large field of view or cellular resolution, but not both. Here, we demonstrate a multimodality imaging platform of optical coherence tomography (OCT) techniques for high resolution, wide field of view in vivo imaging of nanoparticles. This platform includes the first in vivo images of nanoparticle pharmacokinetics acquired with photothermal OCT (PTOCT), along with overlaying images of microvascular and tissue morphology. Gold nanorods (51.8 ± 8.1 nm by 15.2 ± 3.3 nm) were intravenously injected into mice, and their accumulation into mammary tumors was non-invasively imaged in vivo in three dimensions over 24 hours using PTOCT. Spatial frequency analysis of PTOCT images indicated that gold nanorods reached peak distribution throughout the tumors by 16 hours, and remained well-dispersed up to 24 hours post-injection. In contrast, the overall accumulation of gold nanorods within the tumors peaked around 16 hours post-injection. The accumulation of gold nanorods within the tumors was validated post-mortem with multiphoton microscopy. This shows the utility of PTOCT as part of a powerful multimodality imaging platform for the development of nanomedicines and drug delivery technologies.
目前能够在体内追踪纳米颗粒的成像技术，要么提供大视野，要么提供细胞分辨率，但无法两者兼顾。在此，我们展示了一种光学相干断层扫描（OCT）技术的多模态成像平台，用于纳米颗粒的高分辨率、大视野体内成像。该平台包括通过光热OCT（PTOCT）获得的纳米颗粒药代动力学的首张体内图像，以及微血管和组织形态的叠加图像。将金纳米棒（长51.8±8.1纳米，宽15.2±3.3纳米）静脉注射到小鼠体内，并使用PTOCT在24小时内对其在乳腺肿瘤中的积累进行三维无创体内成像。PTOCT图像的空间频率分析表明，金纳米棒在16小时时在整个肿瘤中达到分布峰值，并在注射后24小时内保持良好分散。相比之下，肿瘤内金纳米棒的总体积累在注射后约16小时达到峰值。注射后通过多光子显微镜在死后验证了肿瘤内金纳米棒的积累情况。这表明PTOCT作为强大的多模态成像平台的一部分，对于纳米医学和药物递送技术的发展具有实用性。
