Khezri Maryam, Asghari Zakaria Rasool, Zare Nasser, Johari-Ahar Mohammad
Department of Crop Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
Department of Medicinal Chemistry, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran.
AMB Express. 2022 Jun 3;12(1):65. doi: 10.1186/s13568-022-01409-7.
Galega officinalis L. is an herbaceous legume used to treat symptoms associated with hyperglycemia or diabetes mellitus because of its dominant alkaloid, galegine. In this study, we induced hairy roots in this plant using Rhizobium rhizogenes strain A4, and investigated the effect of type, concentration, and duration of elicitor application on galegine content and some phytochemical characteristics in the hairy roots. Hence, the best growing hairy root line in terms of growth rate was selected and subcultured for treatment with elicitors. Then, at the end of the log phase of growth, chitosan (100, 200, and 400 mg/L), salicylic acid (100, 200, and 300 mM), and ultrasound (1, 2, and 4 min) were applied to hairy roots culture medium. High-performance liquid chromatography (HPLC) showed that the content of galegine was significantly increased after elicitation compared with the control. Thus, the highest content of galegine (14.55 mg/g FW) was obtained 2 days after elicitation when ultrasonic waves were applied to the hairy root culture medium for 4 min. Also, elicitation resulted in a significant increase in the content of total phenol, flavonoid, HO and MDA compared with the control. So that the highest total flavonoid content was obtained in hairy roots that were treated with ultrasonic waves for 4 min and harvested 2 days after elicitation; while, application of 400 mg/L chitosan for 4 days resulted in the highest total phenol (16.84 mg/g FW).
山羊豆是一种豆科草本植物，因其主要生物碱山羊豆碱而被用于治疗与高血糖或糖尿病相关的症状。在本研究中，我们使用发根农杆菌A4菌株诱导该植物产生毛状根，并研究了诱导子类型、浓度和处理时间对毛状根中山羊豆碱含量及一些植物化学特性的影响。因此，选择了生长速率最佳的毛状根系进行继代培养并用诱导子处理。然后，在生长对数期结束时，将壳聚糖（100、200和400 mg/L）、水杨酸（100、200和300 mM）和超声波（1、2和4分钟）应用于毛状根培养基。高效液相色谱（HPLC）显示，与对照相比，诱导后山羊豆碱含量显著增加。因此，当对毛状根培养基施加4分钟超声波后，诱导2天后获得了最高的山羊豆碱含量（14.55 mg/g鲜重）。此外，与对照相比，诱导导致总酚、黄酮、HO和丙二醛含量显著增加。在用4分钟超声波处理并在诱导后2天收获的毛状根中获得了最高的总黄酮含量；而施加400 mg/L壳聚糖4天导致了最高的总酚含量（16.84 mg/g鲜重）。
