Department of Orthopaedics, The First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei, 230022, Anhui, China.
Hum Cell. 2021 Jan;34(1):76-85. doi: 10.1007/s13577-020-00440-9. Epub 2020 Sep 27.
Osteoarthritis (OA) is a chronic musculoskeletal degeneration disease that can result in chronic pain and functional disability. Circular RNAs (CirRNAs) are known to be involved in OA. It was reported that hsa_circ_0037658 was notably upregulated in OA tissues; however, the biological role of hsa_circ_0037658 in OA remains unclear. To investigate the function of hsa_circ_0037658 in OA, CHON-001 cells were treated with IL-1β. The effect of hsa_circ_0037658 knockdown on cell growth was tested by CCK-8 and immunofluorescence staining. In addition, the correlation between hsa_circ_0037658 and autophagy was explored by LC3 staining and western blot. The results indicated that hsa_circ_0037658 was significantly upregulated in IL-1β-treated CHON-001 cells. The silencing of hsa_circ_0037658 could protect CHON-001 cell injury against IL-1β. Moreover, hsa_circ_0037658 shRNA reversed IL-1β-induced cell growth inhibition via inducing cell autophagy. Furthermore, knockdown of hsa_circ_0037658 notably alleviated the symptom of OA in vivo. To sum up, knockdown of hsa_circ_0037658 suppressed the progression of OA via inducing autophagy. Thus, hsa_circ_0037658 might serve as a potential target for the treatment of OA.
骨关节炎（OA）是一种慢性肌肉骨骼退行性疾病，可导致慢性疼痛和功能障碍。已知环状 RNA（CirRNAs）参与 OA 的发生。据报道，hsa_circ_0037658 在 OA 组织中显著上调；然而，hsa_circ_0037658 在 OA 中的生物学作用尚不清楚。为了研究 hsa_circ_0037658 在 OA 中的功能，用 IL-1β 处理 CHON-001 细胞。通过 CCK-8 和免疫荧光染色检测 hsa_circ_0037658 敲低对细胞生长的影响。此外，通过 LC3 染色和 Western blot 探讨 hsa_circ_0037658 与自噬的相关性。结果表明，hsa_circ_0037658 在 IL-1β 处理的 CHON-001 细胞中显著上调。hsa_circ_0037658 的沉默可以保护 CHON-001 细胞免受 IL-1β 的损伤。此外，hsa_circ_0037658 shRNA 通过诱导细胞自噬逆转了 IL-1β 诱导的细胞生长抑制。此外，hsa_circ_0037658 的敲低显著缓解了 OA 的体内症状。总之，hsa_circ_0037658 通过诱导自噬抑制 OA 的进展。因此，hsa_circ_0037658 可能成为治疗 OA 的潜在靶点。
