Chongqing Academy of Animal Sciences, Rongchang, Chongqing, 402460, China.
Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Rongchang, Chongqing, 402460, China.
Genes Genomics. 2020 Nov;42(11):1327-1338. doi: 10.1007/s13258-020-00998-6. Epub 2020 Sep 26.
Lactobacillus reuteri I5007 possesses many excellent probiotic characteristics in piglets. miRNA plays important role in host-microbiota interactions, but the mechanism by which L. reuteri I5007 regulates intestinal function through its influence on miRNA expression is unknown.
This study analyzed the miRNA expression patterns in the ileum mucosa tissue of piglets by L. reuteri I5007 treatment, aim to clarify its molecular mechanism for regulating intestinal function through miRNA.
Neonatal piglets were orally administered L. reuteri I5007 or a placebo daily starting on day 1, and differential expression of ileal miRNAs was analyzed at 10 and 20 days of age by small RNA sequencing.
361 known porcine miRNAs were identified, and ten miRNAs were highly expressed in the ileum mucosa in both treatments. Nineteen differentially expressed (DE) miRNAs were identified in response to L. reuteri treatment, and four DE miRNAs (ssc-miR-196a, -196b-5p, -1285 and -10386) were differentially expressed at both time points. The KEGG pathway analyses showed the targets of 19 DE miRNAs were involved in 63 significantly enriched pathways, including the PI3K-Akt and MAPK pathways, which were confirmed to play important roles in probiotic-host communication. L. reuteri I5007 exerted anti-inflammatory effects by influencing the levels of inflammatory cytokines. Suppressor of cytokine signalling 4 gene was the target gene of ssc-miR-196a/-196b-5p, overexpression of ssc-miR-196a/-196b-5p downregulated the mRNA expression of IL-1β and TNFα in IPEC-J2 cells.
Our study provides new insight into the role of miRNAs in the intestinal function of piglets after L. reuteri I5007 treatment.
鼠李糖乳杆菌 I5007 在仔猪中具有许多优秀的益生菌特性。miRNA 在宿主-微生物相互作用中发挥重要作用，但 L. reuteri I5007 通过影响 miRNA 表达来调节肠道功能的机制尚不清楚。
本研究通过 L. reuteri I5007 处理分析仔猪回肠黏膜组织中的 miRNA 表达谱，旨在阐明其通过 miRNA 调节肠道功能的分子机制。
新生仔猪从第 1 天开始每天口服给予 L. reuteri I5007 或安慰剂，分别在 10 天和 20 天龄时通过小 RNA 测序分析回肠中 miRNA 的差异表达。
鉴定出 361 个已知的猪 miRNA，两种处理均在回肠黏膜中高度表达 10 个 miRNA。L. reuteri 处理后鉴定出 19 个差异表达（DE）miRNA，其中 4 个 DE miRNA（ssc-miR-196a、-196b-5p、-1285 和-10386）在两个时间点均有差异表达。KEGG 通路分析显示，19 个 DE miRNA 的靶基因参与了 63 个显著富集的通路，包括 PI3K-Akt 和 MAPK 通路，这些通路被证实对益生菌-宿主通讯有重要作用。L. reuteri I5007 通过影响炎症细胞因子的水平发挥抗炎作用。细胞因子信号转导抑制因子 4 基因是 ssc-miR-196a/-196b-5p 的靶基因，ssc-miR-196a/-196b-5p 的过表达下调了 IPEC-J2 细胞中 IL-1β 和 TNFα 的 mRNA 表达。
本研究为 L. reuteri I5007 处理后仔猪肠道功能中 miRNA 的作用提供了新的见解。
