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微小RNA-382-5p靶向核受体亚家族3组C成员1以调节慢性不可预测轻度应激诱导的大鼠抑郁样行为。

MicroRNA-382-5p Targets Nuclear Receptor Subfamily 3 Group C Member 1 to Regulate Depressive-Like Behaviors Induced by Chronic Unpredictable Mild Stress in Rats.

作者信息

Li Shuqian, Ma Hong, Yuan Xu, Zhou Xiaomei, Wan Yiwen, Chen Shangjie

机构信息

Department of Rehabilitation, People's Hospital of Shenzhen Baoan District, Shenzhen 518100, People's Republic of China.

Department of Rehabilitation, Binzhou Medical University, Yantai, Shandong Province 264003, People's Republic of China.

出版信息

Neuropsychiatr Dis Treat. 2020 Sep 9;16:2053-2061. doi: 10.2147/NDT.S243920. eCollection 2020.

DOI:10.2147/NDT.S243920
PMID:32982244
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7490101/
Abstract

BACKGROUND

Depression is an emotional disorder characterized by depression, lack of pleasure, and cognitive and sleep disorders. It is a systemic disease with a complex pathogenesis. In this study, we will be focused to investigate their associations and the exact functional mechanisms of miR-382-5p and NR3C1 in depression.

MATERIALS AND METHODS

We measured the expressions of microRNA-382-5p (miR-382-5p) and NR3C1 in the hippocampus by chronic unpredictable mild stress (CUMS). Depression behavior test including novelty-suppressed feeding test (NSFT), sucrose preference test (SPT), and forced swim test (FST) on rats have been conducted to examine the roles and functions of miR-382-5p and NR3C1 on depression-like behaviors by lentivirus vectors.

RESULTS

Up-regulation of miR-382-5p and down-regulation of NR3C1 were observed in rats' hippocampus induced by CUMS. miR-382-5p targeted NR3C1 and inhibited the expressions of NR3C1 in rats' hippocampus. miR-382-5p could significantly change the depression behaviors induced by CUMS. NR3C1 downstream BDNF and p-TrkB were also oppositely associated with miR-382-5p in rats' hippocampus.

CONCLUSION

Through our experiments and analysis, we found that the associations between miR-382-5p and NR3C1 could affect the depression-like behaviors.

摘要

背景

抑郁症是一种以情绪低落、快感缺乏以及认知和睡眠障碍为特征的情感障碍。它是一种发病机制复杂的全身性疾病。在本研究中，我们将着重探讨miR - 382 - 5p和NR3C1在抑郁症中的关联及其确切的功能机制。

材料与方法

我们通过慢性不可预测温和应激（CUMS）来检测大鼠海马体中微小RNA - 382 - 5p（miR - 382 - 5p）和NR3C1的表达。通过慢病毒载体对大鼠进行包括新奇抑制摄食试验（NSFT）、蔗糖偏好试验（SPT）和强迫游泳试验（FST）在内的抑郁行为测试，以研究miR - 382 - 5p和NR3C1在抑郁样行为中的作用和功能。

结果

在CUMS诱导的大鼠海马体中观察到miR - 382 - 5p上调和NR3C1下调。miR - 382 - 5p靶向NR3C1并抑制大鼠海马体中NR3C1的表达。miR - 382 - 5p可显著改变CUMS诱导的抑郁行为。在大鼠海马体中，NR3C1下游的BDNF和p - TrkB也与miR - 382 - 5p呈相反关联。

结论

通过我们的实验和分析，我们发现miR - 382 - 5p与NR3C1之间的关联可能影响抑郁样行为。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad1d/7490101/c336b412a260/NDT-16-2053-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad1d/7490101/714dc7431d77/NDT-16-2053-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad1d/7490101/f7b90fd93a36/NDT-16-2053-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad1d/7490101/4ff574d888d5/NDT-16-2053-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad1d/7490101/eae945cf036e/NDT-16-2053-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad1d/7490101/9cd22efad850/NDT-16-2053-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad1d/7490101/c336b412a260/NDT-16-2053-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad1d/7490101/714dc7431d77/NDT-16-2053-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad1d/7490101/f7b90fd93a36/NDT-16-2053-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad1d/7490101/4ff574d888d5/NDT-16-2053-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad1d/7490101/eae945cf036e/NDT-16-2053-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad1d/7490101/9cd22efad850/NDT-16-2053-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad1d/7490101/c336b412a260/NDT-16-2053-g0006.jpg

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