Peng Ying, Zhan Xiang-Xiang, Cao Yi, Zhang Han-Wen, Cao Wei-Han, Su Yan-Jun, Diao Chang, Sun Qiang-Ming, Cheng Ruo-Chuan
Kunming Medical University of Yunnan Province, Kunming, Yunnan 650500, People's Republic of China.
Thyroid Disease Diagnosis and Treatment Center, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, People's Republic of China.
Onco Targets Ther. 2020 Aug 25;13:8677-8689. doi: 10.2147/OTT.S261685. eCollection 2020.
Thomsen-Friedenreich antibody (TF-Ab) is a specific antibody against the Thomsen-Friedenreich antigen (TF-Ag). At present, studies on a number of other tumors have shown that TF-Ab can effectively inhibit metastasis and induce apoptosis in tumor cells. However, the role of TF-Ab in thyroid cancer (TC) remains unclear.
Normal subjects and patients with primary papillary TC with or without lymph node metastasis were tested for TF-Ab expression by enzyme-linked immunosorbent assays (ELISAs). Immunofluorescence was used to assess the expression of TF-Ag in thyroid papillary carcinoma with or without lymph node metastasis and undifferentiated cancer tissues. To evaluate the role of TF-Ab in TC, the effects of TF monoclonal antibody (mAb A78-G/A7) on cell biological function were investigated by MTT assays, flow cytometry, adhesion assays and transwell experiments.
Compared with normal individuals, TF-Ab levels in patients with TC were decreased, but no changes were observed with respect to lymph node metastasis. The expression of TF-Ag in TC tissues was relatively higher than that detected in adjacent tissues, but it was not affected by the presence or absence of lymph node metastasis. Upon treatment mAb A78-G/A7 treating, TC cell cycles were affected, meanwhile the abilities to adhere, invade and migrate were also significantly reduced.
The results of the present study showed that mAb A78-G/A7 could affect the invasion and migration of all assayed TC cell lines. The effects of mAb A78-G/A7 on the cell cycle, adhesion, invasion and migration of TC cells were more significant than those observed for proliferation and apoptosis.
汤姆森-弗里德赖希抗体（TF-Ab）是一种针对汤姆森-弗里德赖希抗原（TF-Ag）的特异性抗体。目前，对其他多种肿瘤的研究表明，TF-Ab可有效抑制肿瘤细胞转移并诱导其凋亡。然而，TF-Ab在甲状腺癌（TC）中的作用仍不清楚。
采用酶联免疫吸附测定（ELISA）检测正常受试者以及有或无淋巴结转移的原发性乳头状TC患者的TF-Ab表达。利用免疫荧光评估有或无淋巴结转移的甲状腺乳头状癌及未分化癌组织中TF-Ag的表达。为评估TF-Ab在TC中的作用，通过MTT法、流式细胞术、黏附实验和Transwell实验研究TF单克隆抗体（mAb A78-G/A7）对细胞生物学功能的影响。
与正常个体相比，TC患者的TF-Ab水平降低，但淋巴结转移情况未观察到变化。TC组织中TF-Ag的表达相对高于相邻组织中检测到的表达，但不受淋巴结转移与否的影响。用mAb A78-G/A7处理后，TC细胞周期受到影响，同时黏附、侵袭和迁移能力也显著降低。
本研究结果表明，mAb A78-G/A7可影响所有检测的TC细胞系的侵袭和迁移。mAb A78-G/A7对TC细胞周期、黏附、侵袭和迁移的影响比对增殖和凋亡的影响更显著。
