长链非编码RNA LINC01089通过海绵吸附miR-145-5p介导SOX9表达促进胃癌发展。

Long Non-Coding RNA LINC01089 Enhances the Development of Gastric Cancer by Sponging miR-145-5p to Mediate SOX9 Expression.

作者信息

Wang Fengyong, Yang Qiong

机构信息

Department of General Surgery, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, People's Republic of China.

Department of Gastroenteropancreatic Surgery, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, People's Republic of China.

出版信息

Onco Targets Ther. 2020 Sep 16;13:9213-9224. doi: 10.2147/OTT.S249392. eCollection 2020.

BACKGROUND

Long non-coding RNAs (lncRNAs) have potential regulatory effects in oncogenesis. Previous studies showed that several lncRNAs could participate in the progression of gastric cancer (GC). However, the specific biological mechanisms in GC are still unclear. We analyzed an lncRNA microarray of GC and selected LINC01089 for study.

METHODS

LINC01089 expression in GC was tested by qRT-PCR. GC cell proliferation was assessed using CCK-8 and EdU assays. Cell invasion was assessed using the Transwell assay. A dual-luciferase reporter gene assay and bioinformatics assay were performed to detect potential targets of LINC01089. Additionally, RNA immunoprecipitation and Western blot assays were performed to clarify their interactions and roles in the regulation of GC progression.

RESULTS

High LINC01089 expression was observed in GC cells. LINC01089 overexpression notably expedited cell migration, proliferation, and invasion. LINC01089 positively regulated expression by competitively binding to microRNA (miR-145-5p).

CONCLUSION

LINC01089 competitively binds to miR-145-5p to mediate expression. LINC01089 may participate in the progression of GC.

背景

长链非编码RNA（lncRNAs）在肿瘤发生过程中具有潜在的调控作用。先前的研究表明，几种lncRNAs可参与胃癌（GC）的进展。然而，GC中具体的生物学机制仍不清楚。我们分析了GC的lncRNA芯片，并选择LINC01089进行研究。

方法

采用qRT-PCR检测GC中LINC01089的表达。使用CCK-8和EdU检测评估GC细胞增殖。使用Transwell检测评估细胞侵袭。进行双荧光素酶报告基因检测和生物信息学检测以检测LINC01089的潜在靶点。此外，进行RNA免疫沉淀和蛋白质印迹检测以阐明它们在GC进展调控中的相互作用和作用。