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LINC00894 通过吸附 miR-429 调控 ZEB1 表达促进乳腺癌进展。

LINC00894 Enhances the Progression of Breast Cancer by Sponging miR-429 to Regulate ZEB1 Expression.

作者信息

Meng De-Feng, Shao Hua, Feng Chuan-Bo

机构信息

Department of Oncology Surgery, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei, People's Republic of China.

Department of Thyroid and Breast Surgery, The Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu, People's Republic of China.

出版信息

Onco Targets Ther. 2021 May 25;14:3395-3407. doi: 10.2147/OTT.S277284. eCollection 2021.

DOI:10.2147/OTT.S277284
PMID:34079285
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8164724/
Abstract

PURPOSE

Long non-coding RNAs (lncRNAs) are known to regulate tumorigenesis. Although breast cancer tissues show a high expression of , its specific biological role in breast cancer progression is still unknown. In this study, lncRNA microarray was used to analyze the lncRNA expression in breast cancer tissues, and was selected for further analysis.

MATERIALS AND METHODS

Expression of in 45 pairs of breast cancer tissues and normal tissues obtained from patients with breast cancer was assessed by quantitative reverse transcription-PCR, while proliferation and invasion of breast cancer cells were assessed using a Cell Counting Kit-8 (CCK-8), EdU assay, colony formation experiment, and transwell assays. A dual-luciferase reporter gene assay and bioinformatics analysis were employed to detect potential targets of . Additionally, RNA Binding Protein Immunoprecipitation (RIP) and Western blot assays were utilized to clarify its interaction and roles in the regulation of breast cancer progression.

RESULTS

High expression of was observed in breast cancer cells, and its overexpression significantly expedited cell proliferation and invasion. Moreover, positively regulated the expression of by competitively binding to miR-429.

CONCLUSION

Taken together, these results suggest that competitively binds to miR-429 to mediate expression; consequently, it is implicated to play a role in the progression of breast cancer.

摘要

目的

已知长链非编码RNA（lncRNAs）可调节肿瘤发生。尽管乳腺癌组织中显示出 的高表达，但其在乳腺癌进展中的具体生物学作用仍不清楚。在本研究中，使用lncRNA微阵列分析乳腺癌组织中的lncRNA表达，并选择 进行进一步分析。

材料与方法

通过定量逆转录PCR评估45对来自乳腺癌患者的乳腺癌组织和正常组织中 的表达，同时使用细胞计数试剂盒-8（CCK-8）、EdU检测、集落形成实验和transwell检测评估乳腺癌细胞的增殖和侵袭。采用双荧光素酶报告基因检测和生物信息学分析来检测 的潜在靶点。此外，利用RNA结合蛋白免疫沉淀（RIP）和蛋白质免疫印迹检测来阐明其在调节乳腺癌进展中的相互作用和作用。

结果

在乳腺癌细胞中观察到 的高表达，其过表达显著加速细胞增殖和侵袭。此外， 通过竞争性结合miR-429正向调节 的表达。

结论

综上所述，这些结果表明 竞争性结合miR-429以介导 的表达；因此，它与乳腺癌进展有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/e1def2ea1b90/OTT-14-3395-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/6bccc77282be/OTT-14-3395-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/1f5e8eac9794/OTT-14-3395-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/b257ab944fa3/OTT-14-3395-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/7162a31532df/OTT-14-3395-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/d04a8359c18e/OTT-14-3395-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/e785d9929f8f/OTT-14-3395-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/e1def2ea1b90/OTT-14-3395-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/6bccc77282be/OTT-14-3395-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/1f5e8eac9794/OTT-14-3395-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/b257ab944fa3/OTT-14-3395-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/7162a31532df/OTT-14-3395-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/d04a8359c18e/OTT-14-3395-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/e785d9929f8f/OTT-14-3395-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b713/8164724/e1def2ea1b90/OTT-14-3395-g0007.jpg

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