LINC00894 Enhances the Progression of Breast Cancer by Sponging miR-429 to Regulate ZEB1 Expression.

PURPOSE

Long non-coding RNAs (lncRNAs) are known to regulate tumorigenesis. Although breast cancer tissues show a high expression of , its specific biological role in breast cancer progression is still unknown. In this study, lncRNA microarray was used to analyze the lncRNA expression in breast cancer tissues, and was selected for further analysis.

MATERIALS AND METHODS

Expression of in 45 pairs of breast cancer tissues and normal tissues obtained from patients with breast cancer was assessed by quantitative reverse transcription-PCR, while proliferation and invasion of breast cancer cells were assessed using a Cell Counting Kit-8 (CCK-8), EdU assay, colony formation experiment, and transwell assays. A dual-luciferase reporter gene assay and bioinformatics analysis were employed to detect potential targets of . Additionally, RNA Binding Protein Immunoprecipitation (RIP) and Western blot assays were utilized to clarify its interaction and roles in the regulation of breast cancer progression.

RESULTS

High expression of was observed in breast cancer cells, and its overexpression significantly expedited cell proliferation and invasion. Moreover, positively regulated the expression of by competitively binding to miR-429.

CONCLUSION

Taken together, these results suggest that competitively binds to miR-429 to mediate expression; consequently, it is implicated to play a role in the progression of breast cancer.