[植物名称]原球茎的抗氧化、抗癌及抗菌作用

Antioxidant, anticancer and antimicrobial effects of developed protocorms of .

作者信息

Paudel Mukti R, Joshi Pusp R, Chand Krishna, Sah Anil K, Acharya Sameer, Pant Basant, Pant Bijaya

机构信息

Central Department of Botany, Tribhuvan University, Kathmandu, Nepal.

Annapurna Research Center, Kathmandu, Nepal.

出版信息

Biotechnol Rep (Amst). 2020 Sep 7;28:e00527. doi: 10.1016/j.btre.2020.e00527. eCollection 2020 Dec.

DOI:10.1016/j.btre.2020.e00527

PMID:32983924

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7494665/

Abstract

seed germination and protocorms formation were successfully established in traditionally used . Fresh protocorms (178.34 g - 183.90 g) were produced on the elicitor of sp, and supplemented MS-medium. Methanol extract of protocorms has scavenged 94.31 % of DPPH radicals at 1000 μg/mL. Its 117.56 μg/mL concentration has scavenged 50 % DPPH radical (IC). Similarly, it inhibits 25.39 % and 27.80 % HeLa and U251 cells at 500 μg/mL. The IC was found as 350.06 μg/mL and 507.22 μg/mL for HeLa and U251 cells respectively. Further, it inhibited the growth of , and with the zone of inhibition 4, 2 and 2 mm respectively. In conclusion, protocorms developed through seeds culture have accumulated and synthesized bioactive secondary metabolites. Therefore, protocorms could be utilized to the isolation of compounds for formulation of herbal drugs without damaging natural populations.

摘要

在传统使用的方法中成功建立了种子萌发和原球茎形成。在添加了sp诱导剂的改良MS培养基上产生了新鲜原球茎（178.34 g - 183.90 g）。原球茎的甲醇提取物在1000μg/mL时清除了94.31%的DPPH自由基。其浓度为117.56μg/mL时清除了50%的DPPH自由基（IC）。同样，在500μg/mL时，它对HeLa和U251细胞的抑制率分别为25.39%和27.80%。HeLa和U251细胞的IC分别为350.06μg/mL和507.22μg/mL。此外，它对[具体细菌名称未给出]、[具体细菌名称未给出]和[具体细菌名称未给出]的生长有抑制作用，抑菌圈分别为4、2和2 mm。总之，通过种子培养发育的原球茎积累并合成了生物活性次生代谢物。因此，原球茎可用于分离化合物以制备草药制剂，而不会损害自然种群。