Remmelzwaal Sanne, Boxem Mike
Developmental Biology, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, the Netherlands.
Curr Opin Syst Biol. 2019 Feb;13:1-9. doi: 10.1016/j.coisb.2018.08.006.
The systematic identification of all protein-protein interactions that take place in an organism (the 'interactome') is an important goal in modern biology. The nematode was one of the first multicellular models for which a proteome-wide interactome mapping project was initiated. Most interactome mapping efforts have utilized the yeast two-hybrid system, yielding an extensive binary interactome, while recent developments in mass spectrometry-based approaches hold great potential for further improving our understanding of protein interactome networks in a multicellular context. For example, methods like co-fractionation, proximity labeling, and tissue-specific protein purification not only identify protein-protein interactions, but have the potential to provide crucial insight into when and where interactions take place. Here we review current standards and recent improvements in protein interaction mapping in
系统鉴定生物体中发生的所有蛋白质-蛋白质相互作用（“相互作用组”）是现代生物学的一个重要目标。线虫是最早启动全蛋白质组相互作用组图谱绘制项目的多细胞模型之一。大多数相互作用组图谱绘制工作都利用了酵母双杂交系统，产生了广泛的二元相互作用组，而基于质谱方法的最新进展对于进一步增进我们在多细胞环境中对蛋白质相互作用网络的理解具有巨大潜力。例如，共分级分离、邻近标记和组织特异性蛋白质纯化等方法不仅能鉴定蛋白质-蛋白质相互作用，还有可能提供关于相互作用发生的时间和地点的关键见解。在此，我们综述了蛋白质相互作用图谱绘制的当前标准和近期进展。
