人类冠状病毒的实验室检测方法。

Laboratory detection methods for the human coronaviruses.

机构信息

Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.

Yeates School of Graduate Studies, Ryerson University, Toronto, ON, Canada.

出版信息

Eur J Clin Microbiol Infect Dis. 2021 Feb;40(2):225-246. doi: 10.1007/s10096-020-04001-8. Epub 2020 Sep 28.

Abstract

Coronaviruses are a group of envelop viruses which lead to diseases in birds and mammals as well as human. Seven coronaviruses have been discovered in humans that can cause mild to lethal respiratory tract infections. HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1 are the low-risk members of this family and the reason for some common colds. Besides, SARS-CoV, MERS-CoV, and newly identified SARS-CoV-2, which is also known as 2019-nCoV, are the more dangerous viruses. Due to the rapid spread of this novel coronavirus and its related disease, COVID-19, a reliable, simple, fast, and low-cost detection method is necessary for patient diagnosis and tracking worldwide. Human coronaviruses detection methods were classified and presented in this article. The laboratory detection techniques include RT-PCR, RT-LAMP, electrochemical and optical biosensors for RNA detection, and whole virus or viral proteins detection assays.

摘要

冠状病毒是一组包膜病毒,可导致鸟类和哺乳动物以及人类患病。已在人类中发现七种可引起轻度至致命呼吸道感染的冠状病毒。HCoV-229E、HCoV-OC43、HCoV-NL63 和 HCoV-HKU1 是该家族的低风险成员,也是引起普通感冒的原因。此外,SARS-CoV、MERS-CoV 和新发现的严重急性呼吸综合征冠状病毒 2 型(也称为 2019-nCoV)是更危险的病毒。由于这种新型冠状病毒及其相关疾病 COVID-19 的迅速传播,全球范围内有必要建立一种可靠、简单、快速和低成本的检测方法,用于患者诊断和追踪。本文对人类冠状病毒的检测方法进行了分类和介绍。实验室检测技术包括用于 RNA 检测的 RT-PCR、RT-LAMP、电化学和光学生物传感器以及全病毒或病毒蛋白检测分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddd0/7520381/9a396a060c1b/10096_2020_4001_Fig1_HTML.jpg

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