Department of Zoology, Mizoram University, Aizawl, Mizoram 796 004, India.
Department of Zoology, Mizoram University, Aizawl, Mizoram 796 004, India.
J Steroid Biochem Mol Biol. 2020 Nov;204:105763. doi: 10.1016/j.jsbmb.2020.105763. Epub 2020 Sep 25.
Pubertal ovarian function might be dependent on the factors present in the pre-pubertal stages. Visfatin regulates ovarian steroidogenesis in adult. To date, no study has investigated the role of visfatin either in pre-pubertal or pubertal mice ovary. Thus, we investigated the role of visfatin in pre-pubertal mice ovary in relation to steroidogenesis and proliferation and apoptosis in vitro by inhibiting the endogenous visfatin by a specific inhibitor, FK866. Inhibition of visfatin increased the estrogen secretion and also up-regulated the expression of CYP11A1, 17βHSD and CYP19A1 in mice ovary. Furthermore, active caspase3 was up-regulated along with the down-regulation of BAX and BCL2 in the pre-pubertal ovary after visfatin inhibition. The expression of GCNA, PCNA, and BrdU labeling was also decreased by FK866 treatment. These results suggest that visfatin inhibits steroidogenesis, increases proliferation, and suppresses apoptosis in the pre-pubertal mice ovary. So, visfatin is a new regulator of ovary function in pre-pubertal mice.
青春期卵巢功能可能依赖于青春期前阶段存在的因素。内脂素在成年后调节卵巢甾体生成。迄今为止，尚无研究调查内脂素在青春期前或青春期小鼠卵巢中的作用。因此，我们通过使用特异性抑制剂 FK866 抑制内源性内脂素，在体外研究内脂素在青春期前小鼠卵巢中的作用及其与甾体生成、增殖和凋亡的关系。内脂素抑制增加了雌激素分泌，并上调了 CYP11A1、17βHSD 和 CYP19A1 在小鼠卵巢中的表达。此外，在青春期前卵巢中内脂素抑制后，活性 caspase3 上调，同时 BAX 和 BCL2 下调。GCNA、PCNA 和 BrdU 标记的表达也因 FK866 处理而减少。这些结果表明，内脂素抑制青春期前小鼠卵巢中的甾体生成、增加增殖并抑制凋亡。因此，内脂素是青春期前小鼠卵巢功能的新调节剂。
