蛋白水解对重组染色质转录保真度的影响。
Effect of proteolysis of transcriptional fidelity of reconstituted chromatin.
作者信息
Gadski R A, Chae C B
出版信息
Biochemistry. 1977 Jul 26;16(15):3465-9. doi: 10.1021/bi00634a027.
The effect of proteolysis on the transcriptional properties of reconstituted rat liver chromatin was studied. Within the sensitivity of currently available methods, proteolysis of chromosomal proteins by chromatin-bound protease during chromatin reconstitution has no apparent effect on: (1) number of initiation sites, (2) proportion of reiterating and unique sequences of DNA transcribed, (3) size of the RNA transcribed, and (4) transcription of DNA sequences complementary to poly(A) containing messenger RNA.
研究了蛋白水解对重组大鼠肝染色质转录特性的影响。在现有方法的检测灵敏度范围内，染色质重组过程中染色质结合蛋白酶对染色体蛋白的蛋白水解作用对以下方面无明显影响：（1）起始位点数量；（2）转录的DNA重复序列和单一序列的比例；（3）转录的RNA大小；（4）与含多聚腺苷酸的信使RNA互补的DNA序列的转录。
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