Nepal Academy of Science and Technology (NAST), Lalitpur, Nepal.
Everest International Clinic and Research Center, Kathmandu, Nepal.
Trans R Soc Trop Med Hyg. 2021 Jun 2;115(6):619-626. doi: 10.1093/trstmh/traa096.
Dengue cases have been continuously reported in Nepal, including some large outbreaks, since its first introduction in 2004. The disease is now expanding towards newer locations above 1400 m high, especially the country's capital city, Kathmandu. In 2019, >14,000 dengue cases including six deaths were reported. This study was aimed at the detection and molecular characterization of dengue virus (DENV) in dengue patients.
A total of 451 patients were enrolled in this study. Demographic, clinical and laboratory information was collected from dengue patients. Dengue infection was confirmed by antibody/antigen detection assays followed by RT-PCR analysis.
The DENV patients showed fever, body ache, headache, myalgia, retro-orbital pain and arthralgia. The platelets were decreased, serum liver enzymes were increased and leucopenia was seen. Out of 195 patients, 111 (57.0%) were positive for DENV RNA by consensus PCR. We found DENV-2, 70 (63.1%) as the predominant serotype responsible for the 2019 outbreak, while DENV-3 was detected in two patients.
Our findings suggest that DENV-2 was the major serotype causing the 2019 massive outbreak in Nepal. This information will help in disease control programs to understand the molecular epidemiology and its changing trend.
自 2004 年首次引入以来，尼泊尔不断报告登革热病例，包括一些大规模暴发。该疾病现在正朝着海拔 1400 米以上的新地区扩展，特别是该国首都加德满都。2019 年，报告了超过 14000 例登革热病例，包括 6 例死亡。本研究旨在检测和分子特征分析登革热病毒（DENV）在登革热患者中的情况。
本研究共纳入 451 例患者。从登革热患者中收集人口统计学、临床和实验室信息。通过抗体/抗原检测试剂盒确认登革热感染，然后进行 RT-PCR 分析。
DENV 患者表现出发热、身体疼痛、头痛、肌痛、眼眶后疼痛和关节痛。血小板减少、血清肝酶升高和白细胞减少。在 195 例患者中，有 111 例（57.0%）通过共识聚合酶链反应（PCR）对 DENV RNA 呈阳性。我们发现 DENV-2 为主要血清型，导致 2019 年的暴发，有 70 例（63.1%），而 DENV-3 在两名患者中检测到。
我们的研究结果表明，DENV-2 是导致尼泊尔 2019 年大规模暴发的主要血清型。这些信息将有助于疾病控制计划，以了解分子流行病学及其变化趋势。
