尼泊尔 2019 年登革热疫情的分子研究。

Molecular study of 2019 dengue fever outbreaks in Nepal.

机构信息

Nepal Academy of Science and Technology (NAST), Lalitpur, Nepal.

Everest International Clinic and Research Center, Kathmandu, Nepal.

出版信息

Trans R Soc Trop Med Hyg. 2021 Jun 2;115(6):619-626. doi: 10.1093/trstmh/traa096.

DOI:10.1093/trstmh/traa096

PMID:32987406

Abstract

BACKGROUND

Dengue cases have been continuously reported in Nepal, including some large outbreaks, since its first introduction in 2004. The disease is now expanding towards newer locations above 1400 m high, especially the country's capital city, Kathmandu. In 2019, >14,000 dengue cases including six deaths were reported. This study was aimed at the detection and molecular characterization of dengue virus (DENV) in dengue patients.

METHODS

A total of 451 patients were enrolled in this study. Demographic, clinical and laboratory information was collected from dengue patients. Dengue infection was confirmed by antibody/antigen detection assays followed by RT-PCR analysis.

RESULTS

The DENV patients showed fever, body ache, headache, myalgia, retro-orbital pain and arthralgia. The platelets were decreased, serum liver enzymes were increased and leucopenia was seen. Out of 195 patients, 111 (57.0%) were positive for DENV RNA by consensus PCR. We found DENV-2, 70 (63.1%) as the predominant serotype responsible for the 2019 outbreak, while DENV-3 was detected in two patients.

CONCLUSION

Our findings suggest that DENV-2 was the major serotype causing the 2019 massive outbreak in Nepal. This information will help in disease control programs to understand the molecular epidemiology and its changing trend.

摘要

背景

自 2004 年首次引入以来，尼泊尔不断报告登革热病例，包括一些大规模暴发。该疾病现在正朝着海拔 1400 米以上的新地区扩展，特别是该国首都加德满都。2019 年，报告了超过 14000 例登革热病例，包括 6 例死亡。本研究旨在检测和分子特征分析登革热病毒（DENV）在登革热患者中的情况。

方法

本研究共纳入 451 例患者。从登革热患者中收集人口统计学、临床和实验室信息。通过抗体/抗原检测试剂盒确认登革热感染，然后进行 RT-PCR 分析。

结果

DENV 患者表现出发热、身体疼痛、头痛、肌痛、眼眶后疼痛和关节痛。血小板减少、血清肝酶升高和白细胞减少。在 195 例患者中，有 111 例（57.0%）通过共识聚合酶链反应（PCR）对 DENV RNA 呈阳性。我们发现 DENV-2 为主要血清型，导致 2019 年的暴发，有 70 例（63.1%），而 DENV-3 在两名患者中检测到。