Wells M T, Gaffin S L, Jordaan J P
Life Sci. 1987 Jun 29;40(26):2543-50. doi: 10.1016/0024-3205(87)90077-4.
Lethal whole body irradiation damages the gut mucosa and leads to leakage of endotoxin or lipopolysaccharides (LPS) into the systemic circulation. Sixteen rabbits, irradiated with 900 rads 60Co, were randomly divided on day 4 into 2 groups, one of which received an intraperitoneal injection of normal saline (control) and the other (experimental) an equal volume of anti-LPS hyperimmune plasma. The time course of endotoxemia and bacteremia were determined for the duration of the experiment. While rabbits in both groups died within 13 days after irradiation, rabbits given saline died on average 2 days earlier, than rabbits given anti-LPS plasma. Plasma LPS concentrations rose to a small peak on day 2 prior to treatment. Thereafter plasma LPS in rabbits given saline increased forty fold by day 9. In contrast, in rabbits given anti-LPS plasma, LPS concentrations in the plasma remained within pretreatment limits (p 0.01). By day 12 after irradiation, plasma anti-LPS IgG had declined to 5.8% of pretreatment levels in rabbits given saline as compared to 46% in rabbits given anti-LPS plasma (p 0.005). Whilst both groups developed gram-positive bacteremia, rabbits given saline in addition also developed gram-negative bacteremia. No rabbits treated with Anti-LPS showed gram-negative bacteremia. Treatment with Anti-LPS plasma thus significantly protects radiated rabbits from the incidence of gram-negative bacteremia, development of high plasma LPS levels and hence endotoxemia, and prolongs survival to a certain extent.
致死性全身照射会损伤肠道黏膜，导致内毒素或脂多糖（LPS）泄漏至体循环。16只接受900拉德60钴照射的兔子在第4天被随机分为2组，其中一组腹腔注射生理盐水（对照组），另一组（实验组）注射等量的抗LPS超免疫血浆。在实验期间测定内毒素血症和菌血症的时间进程。虽然两组兔子在照射后13天内均死亡，但注射生理盐水的兔子平均比注射抗LPS血浆的兔子早2天死亡。治疗前第2天血浆LPS浓度升至一个小高峰。此后，注射生理盐水的兔子血浆LPS在第9天增加了40倍。相比之下，注射抗LPS血浆的兔子血浆LPS浓度保持在治疗前水平范围内（p<0.01）。照射后第12天，注射生理盐水的兔子血浆抗LPS IgG降至治疗前水平的5.8%，而注射抗LPS血浆的兔子为46%（p<0.005）。虽然两组均发生革兰氏阳性菌血症，但注射生理盐水的兔子还发生了革兰氏阴性菌血症。接受抗LPS治疗的兔子未出现革兰氏阴性菌血症。因此，抗LPS血浆治疗可显著保护受辐射兔子免受革兰氏阴性菌血症的发生、高血浆LPS水平及由此导致的内毒素血症，并在一定程度上延长生存期。
