Bhattacharjee A K, Opal S M, Taylor R, Naso R, Semenuk M, Zollinger W D, Moran E E, Young L, Hammack C, Sadoff J C, Cross A S
Department of Bacterial Diseases, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.
J Infect Dis. 1996 May;173(5):1157-63. doi: 10.1093/infdis/173.5.1157.
Earlier studies showed that purified IgG from sera of rabbits immunized with a boiled Escherichia coli J5 (Rc chemotype) whole cell vaccine protected neutropenic rats against gram-negative bacterial sepsis. In the present study, de-O-acylated J5 lipopolysaccharide (J5 DLPS) as a noncovalent complex with Neisseria meningitidis group B outer membrane protein (GBOMP) elicited anti-J5 LPS antibodies in rabbits. IgG prepared from immune rabbit sera protected neutropenic rats against lethal challenge with Pseudomonas aeruginosa 12:4:4 (Fisher Devlin immunotype 6). Sixteen of 26 rats treated with the postimmune serum IgG were protected compared with none of 20 rats treated with the control rabbit serum IgG (P < .001). In vitro binding studies showed binding of anti-J5 IgG to several gram-negative bacteria. These results indicate that a subunit vaccine made of J5 DLPS as a noncovalent complex with GBOMP may protect against gram-negative bacteremia.
早期研究表明，用煮沸的大肠杆菌J5（Rc化学型）全细胞疫苗免疫的兔血清中纯化的IgG可保护中性粒细胞减少的大鼠免受革兰氏阴性菌败血症的侵害。在本研究中，去O-酰化的J5脂多糖（J5 DLPS）作为与B群脑膜炎奈瑟菌外膜蛋白（GBOMP）的非共价复合物，在兔体内引发了抗J5 LPS抗体。从免疫兔血清中制备的IgG保护中性粒细胞减少的大鼠免受铜绿假单胞菌12:4:4（费舍尔·德夫林免疫型6）的致死性攻击。用免疫后血清IgG治疗的26只大鼠中有16只受到保护，而用对照兔血清IgG治疗的20只大鼠无一受到保护（P <.001）。体外结合研究表明抗J5 IgG与几种革兰氏阴性菌结合。这些结果表明，由J5 DLPS作为与GBOMP的非共价复合物制成的亚单位疫苗可能预防革兰氏阴性菌血症。
