Gan Yi, Chen Hong, Zhou Xian-Rong, Chu Ling-Ling, Ran Wan-Ting, Tan Fang, Zhao Xin
Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, China.
Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing, China.
J Food Biochem. 2020 Nov;44(11):e13495. doi: 10.1111/jfbc.13495. Epub 2020 Sep 28.
Probiotics are regard as safety approaches for preventing and treating some chronic diseases. This study investigated the regulating effect of Lactobacillus plantarum CQPC03 (LP-CQPC03) on lipid metabolism in high-fat diet (HFD)-induced obesity in mice. The results showed that administration of LP-CQPC03 at a concentration of 1.0 × 10 CFU/kg body weight inhibits HFD-induced obesity and improves lipid metabolism in the liver and serum. LP-CQPC03 intervention attenuated obesity-induced hepatic tissue damage, led decreases in hepatic triglyceride (42.02 mmol/gprot), total cholesterol (3.85 mmol/gprot), and LDL-C (1.03 mmol/gprot), and an increase in HDL-C (1.07 mmol/gprot). The same tendencies were observed in serum of HFD-fed mice. LP-CQPC03 intervention led a decrease in serum levels of aspartic transaminase, alanine transaminase, and alkaline phosphatase. LP-CQPC03 alleviated inflammation by increasing the level of interleukin (IL)-4 and IL-10, and decreasing the levels of pro-inflammatory factors, including IL-6, IL-1β, tumor necrosis factor-α, and interferon-γ. LP-CQPC03 also increased activities of SOD and GSH-Px in liver significantly and dropped the hepatic malondialdehyde (MDA) level from 3.39 nmol/gprot to 1.90 nmol/gprot. RT-qPCR results showed that the lipid metabolism-improving effect of LP-CQPC03 was performed by upregulating the expression of carnitine palmitoyltransferase 1, lipoprotein lipase, catalase, and superoxide dismutase 1. This study indicates that L. plantarum CQPC03 might be a potential probiotic that can help mitigate the adverse effects of excessive lipids on the liver, and prevent or alleviate high-energy intake-related obesity. PRACTICAL APPLICATIONS: Intaking high-energy foods is a potential risk of lipid metabolic disorder. Therefore, it is necessary to seek an effective and safe approach for preventing the obesity-related disease. This study found that LP-CQPC03 limited the rate of increase in body weight of mice fed on HFD, maintained normal hepatic tissue morphology, and exhibited a strong regulating effect on lipid metabolism. And the threshold concentration of LP-CQPC03 for the lipid-lowering effect was 1.0 × 10 CFU/kg body weight. Therefore, LP-CQPC03 is a potential probiotic for preventing or alleviating high-energy intake-related obesity.
益生菌被视为预防和治疗某些慢性疾病的安全方法。本研究调查了植物乳杆菌CQPC03（LP-CQPC03）对高脂饮食（HFD）诱导的小鼠肥胖中脂质代谢的调节作用。结果表明，以1.0×10⁹CFU/kg体重的浓度给予LP-CQPC03可抑制HFD诱导的肥胖，并改善肝脏和血清中的脂质代谢。LP-CQPC03干预减轻了肥胖诱导的肝组织损伤，导致肝甘油三酯（42.02 mmol/g蛋白）、总胆固醇（3.85 mmol/g蛋白）和低密度脂蛋白胆固醇（1.03 mmol/g蛋白）水平降低，高密度脂蛋白胆固醇（1.07 mmol/g蛋白）水平升高。在喂食HFD的小鼠血清中也观察到了相同的趋势。LP-CQPC03干预导致血清中天冬氨酸转氨酶、丙氨酸转氨酶和碱性磷酸酶水平降低。LP-CQPC03通过提高白细胞介素（IL）-4和IL-10水平，降低包括IL-6、IL-1β、肿瘤坏死因子-α和干扰素-γ在内的促炎因子水平来减轻炎症。LP-CQPC03还显著提高了肝脏中SOD和GSH-Px的活性，并将肝脏丙二醛（MDA）水平从3.39 nmol/g蛋白降至1.90 nmol/g蛋白。RT-qPCR结果表明，LP-CQPC03对脂质代谢的改善作用是通过上调肉碱棕榈酰转移酶1、脂蛋白脂肪酶、过氧化氢酶和超氧化物歧化酶1的表达来实现的。本研究表明，植物乳杆菌CQPC03可能是一种潜在的益生菌，有助于减轻过量脂质对肝脏的不良影响，并预防或减轻与高能量摄入相关的肥胖。实际应用：摄入高能量食物存在脂质代谢紊乱的潜在风险。因此，有必要寻找一种有效且安全的方法来预防与肥胖相关的疾病。本研究发现，LP-CQPC03限制了喂食HFD的小鼠体重增加的速度，维持了正常的肝组织形态，并对脂质代谢表现出强大的调节作用。LP-CQPC03产生降脂作用的阈值浓度为1.0×10⁹CFU/kg体重。因此，LP-CQPC03是预防或减轻与高能量摄入相关肥胖的潜在益生菌。
