Gan Yi, Tang Ming-Wei, Tan Fang, Zhou Xian-Rong, Fan Ling, Xie Yu-Xin, Zhao Xin
Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, China.
Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing, China.
J Food Biochem. 2020 Dec;44(12):e13491. doi: 10.1111/jfbc.13491. Epub 2020 Oct 1.
Lactic acid bacteria (LAB) are an important group of microorganisms in the food industry. LAB with health benefits are considered natural elements promoting consumer health. The study investigated the anti-obesity effects of Lactobacillus plantarum CQPC01 (LP-CQPC01) on high-fat diet-induced mice. Liver morphology, liver function indexes, lipid indexes, and inflammatory factors in the serum and liver were determined. Expressions of lipid metabolism-related gene were also detected by qPCR. LP-CQPC01 slowed the HFD-induced increase in body weight, decreased the organ indexes, alleviated hepatic lipid accumulation, and inhibited the increased adipose cell volume. LP-CQPC01 decreased lipid levels of serum and liver, and the contents of pro-inflammatory factors, and increased the IL-4 and IL-10 contents. LP-CQPC01 downregulated the expressions of the C/EBP-α and PPARγ mRNA and upregulated CYP7A1, CPT1, LPL, CAT, SOD1, and SOD2 mRNA. Our results indicated that LP-CQPC01 is a potential probiotic for preventing or alleviating high-energy intake-related lipid conditions. PRACTICAL APPLICATIONS: Obesity is a factor of a variety of cardiovascular diseases. Therefore, it is necessary to suppress the occurrence of fat accumulation in time. This study investigated the effect of LP-CQPC01 on lipid regulation in mice fed a high-fat diet (HFD) and clarified the mechanism of strain to alleviate obesity by enhancing the decomposition of cholesterol and detoxification of fat. LP-CQPC01 reduced fat accumulation without oxidative damage, and was confirmed by the attenuated pathological changes of liver. This research can serve as a significant reference for future research, prevention, and treatment of high-energy intake-related lipid conditions, and the development of functional foods with anti-obesity activity.
乳酸菌（LAB）是食品工业中一类重要的微生物。具有健康益处的乳酸菌被视为促进消费者健康的天然元素。本研究调查了植物乳杆菌CQPC01（LP-CQPC01）对高脂饮食诱导的小鼠的抗肥胖作用。测定了肝脏形态、肝功能指标、血脂指标以及血清和肝脏中的炎症因子。还通过qPCR检测了脂质代谢相关基因的表达。LP-CQPC01减缓了高脂饮食诱导的体重增加，降低了器官指数，减轻了肝脏脂质积累，并抑制了脂肪细胞体积的增加。LP-CQPC01降低了血清和肝脏的脂质水平以及促炎因子的含量，并增加了IL-4和IL-10的含量。LP-CQPC01下调了C/EBP-α和PPARγ mRNA的表达，并上调了CYP7A1、CPT1、LPL、CAT、SOD1和SOD2 mRNA的表达。我们的结果表明，LP-CQPC01是预防或缓解与高能量摄入相关的脂质状况的潜在益生菌。实际应用：肥胖是多种心血管疾病的一个因素。因此，有必要及时抑制脂肪积累的发生。本研究调查了LP-CQPC01对高脂饮食（HFD）喂养小鼠脂质调节的影响，并阐明了该菌株通过增强胆固醇分解和脂肪解毒来减轻肥胖的机制。LP-CQPC01减少了脂肪积累而无氧化损伤，肝脏病理变化减轻证实了这一点。本研究可为未来与高能量摄入相关的脂质状况的研究、预防和治疗以及具有抗肥胖活性的功能性食品的开发提供重要参考。
