State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, People's Republic of China.
CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, People's Republic of China.
Emerg Microbes Infect. 2020 Dec;9(1):2269-2277. doi: 10.1080/22221751.2020.1830715.
Replication-competent vesicular stomatitis virus (VSV)-based recombinant viruses are useful tools for studying emerging and highly pathogenic enveloped viruses in level 2 biosafety facilities. Here, we used a replication-competent recombinant VSVs (rVSVs) encoding the spike (S) protein of SARS-CoV-2 in place of the original G glycoprotein (rVSV-eGFP-SARS-CoV-2) to develop a high-throughput entry assay for SARS-CoV-2. The S protein was incorporated into the recovered rVSV-eGFP-SARS-CoV-2 particles, which could be neutralized by sera from convalescent COVID-19 patients. The recombinant SARS-CoV-2 also displayed entry characteristics similar to the wild type virus, such as cell tropism and pH-dependence. The neutralizing titers of antibodies and sera measured by rVSV-eGFP-SARS-CoV-2 were highly correlated with those measured by wild-type viruses or pseudoviruses. Therefore, this is a safe and convenient screening tool for SARS-CoV-2, and it may promote the development of COVID-19 vaccines and therapeutics.
复制型具有感染能力的水疱性口炎病毒（VSV）重组病毒是在二级生物安全设施中研究新兴和高致病性包膜病毒的有用工具。在这里，我们使用编码 SARS-CoV-2 刺突（S）蛋白的复制型具有感染能力的重组 VSV（rVSV）替代原始的 G 糖蛋白（rVSV-eGFP-SARS-CoV-2），开发了一种用于 SARS-CoV-2 的高通量进入检测法。S 蛋白被整合到回收的 rVSV-eGFP-SARS-CoV-2 颗粒中，这些颗粒可以被来自康复期 COVID-19 患者的血清中和。重组 SARS-CoV-2 还表现出与野生型病毒相似的进入特征，例如细胞嗜性和 pH 依赖性。通过 rVSV-eGFP-SARS-CoV-2 测量的抗体和血清的中和滴度与通过野生型病毒或假病毒测量的中和滴度高度相关。因此，这是一种安全便捷的 SARS-CoV-2 筛选工具，它可能会促进 COVID-19 疫苗和疗法的发展。
