Talebi Marjan, Zarshenas Mohammad M, Yazdani Elahe, Moein Mahmoodreza
Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Curr Drug Discov Technol. 2021;18(5):e28092020186381. doi: 10.2174/1570163817666200929114517.
Free radicals can lead to liver dysfunction. Quality control of traditional formulations ensures their safe, pure, and pharmaceutical efficacy. "Qurs-e-Vard", containing petals of Rosa damascena Mill., fruits of Rhus coriaria L. and roots of Glycyrrhiza glabra L. has been suggested as a hepatoprotective preparation in Traditional Persian Medicine (TPM).
This study was directed at the evaluation of the phytochemical characterization, standardization, and in vitro antioxidant activity determination of a solid formulation and its components.
Some qualitative and quantitative controls were performed like ash value, heavy metals investigation, and microbial contamination. The phytochemical assays were used for obtaining total phenolic and flavonoid contents with spectrophotometric methods. 2, 2-Diphenyl-1-c (DPPH) and Nitric Oxide (NO) assays were run for determining Radical scavenging activities of the formulation and its components. Ferric reducing antioxidant power assay (FRAP) was determined as well.
Total phenolic contents of hydroalcoholic and aqueous extracts of the polyherbal formulation measured respectively, (376±0.93) and (297.6±0.96) mg of gallic acid/g of dry matter. Total flavonoid contents of the formulation were also measured (36.27±0.98) for hydroalcoholic extract and (17.79±0.86) mg of quercetin/g of dry matter for aqueous extract. The IC50 of hydroalcoholic and aqueous extract was obtained (88.14±1.15) and (140.78±2.98) μg/ml, respectively. NO scavenging percentages (200μg/ml) of hydroalcoholic and aqueous extracts were measured (59.11±2.15) and (65.08±2.35). FRAP values of hydroalcoholic and aqueous extracts were achieved (255.24±3.45) and (134.57±3.45) μg/ml as well.
Our findings indicated that this polyherbal formulation and its components have justifiable antioxidant effects.
自由基可导致肝功能障碍。传统制剂的质量控制可确保其安全性、纯度和药效。“Qurs-e-Vard”含有大马士革蔷薇花瓣、盐肤木果实和光果甘草根，在传统波斯医学中被认为是一种保肝制剂。
本研究旨在评估一种固体制剂及其成分的植物化学特征、标准化和体外抗氧化活性测定。
进行了一些定性和定量控制，如灰分值、重金属检测和微生物污染检测。采用分光光度法进行植物化学分析，以获得总酚和黄酮含量。采用2,2-二苯基-1-苦基肼（DPPH）和一氧化氮（NO）测定法测定制剂及其成分的自由基清除活性。还测定了铁还原抗氧化能力测定法（FRAP）。
该多草药制剂的水醇提取物和水提取物的总酚含量分别为（376±0.93）和（297.6±0.96）mg没食子酸/g干物质。该制剂的总黄酮含量也分别测定为水醇提取物（36.27±0.98）mg槲皮素/g干物质和水提取物（17.79±0.86）mg槲皮素/g干物质。水醇提取物和水提取物的IC50分别为（88.14±1.15）和（140.78±2.98）μg/ml。水醇提取物和水提取物的NO清除率（200μg/ml）分别为（59.11±2.15）和（65.08±2.35）。水醇提取物和水提取物的FRAP值分别为（255.24±3.45）和（134.57±3.45）μg/ml。
我们的研究结果表明，这种多草药制剂及其成分具有合理的抗氧化作用。
