Memariani Zahra, Hajimahmoodi Mannan, Minaee Bagher, Khodagholi Fariba, Yans Asal, Rahimi Roja, Amin Gholamreza, Moghaddam Ghazaleh, Toliyat Tayebeh, Sharifzadeh Mohammad
Department of Traditional Pharmacy, Faculty of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Pharm Res. 2017 Spring;16(2):694-707.
"VARD" formula consisting of Mill. (Rosaceae) petals, and rhizomes of L. (Papilionaceae) and DC. (Valerianaceae), has been proposed for gastric ulcer in Iranian traditional medicine. We investigated the antiulcer activity of each plant separately and in combination. The biochemical and molecular functions of extracts were also evaluated. Each plant hydroalcoholic extract was standardized via determination of total phenolic and flavonoid contents, also via some phenolic compounds determination and specially glycyrrhizic acid in by using HPLC. Rats received orally extracts of the plants (20, 40 and 80 mg/Kg) and "VARD" (45 mg/Kg) 1 h before ethanol administration. Two h after receiving ethanol, animals were sacrificed; the stomach was removed for macroscopic and microscopic assessment. Also heme-oxygenase-1, glutathione, and catalase were measured in the gastric tissue of the rats pretreated by "VARD" and dose of 20 mg/Kg of extracts. Among three extracts, and contained more total phenolic and flavonoid content respectively. Gallic acid was prominent compound in The extracts of , , and significantly decreased ulcer index. ED values were 8.2, 31.86 and 25.08 mg/Kg respectively. "VARD" significantly decreased ulcer index compared to 20 mg/Kg of ( < 0.0001) and ( < 0.001). Pretreatment with "VARD" and each plant extracts (20 mg/Kg) increased glutathione, catalse and heme-oxygenase-1 significantly ( < 0.05 in comparison with control group. Our findings indicate that "VARD" partly via antioxidant activity can be considered as an effective antiulcer formula.
由蔷薇科植物花瓣、豆科植物根茎和缬草科植物根茎组成的“VARD”配方，在伊朗传统医学中已被用于治疗胃溃疡。我们分别研究了每种植物及其组合的抗溃疡活性。还评估了提取物的生化和分子功能。通过测定总酚和黄酮含量、一些酚类化合物的含量，特别是利用高效液相色谱法测定甘草酸的含量，对每种植物的水醇提取物进行标准化。大鼠在给予乙醇前1小时口服植物提取物（20、40和80毫克/千克）和“VARD”（45毫克/千克）。给予乙醇2小时后，处死动物；取出胃进行宏观和微观评估。还测定了用“VARD”预处理以及剂量为20毫克/千克提取物的大鼠胃组织中的血红素加氧酶-1、谷胱甘肽和过氧化氢酶。在三种提取物中，分别含有更多的总酚和黄酮含量。没食子酸是植物提取物中的主要化合物。植物提取物、和显著降低溃疡指数。半数有效剂量分别为8.2、31.86和25.08毫克/千克。与20毫克/千克的植物提取物和相比，“VARD”显著降低溃疡指数（<0.0001和<0.001）。用“VARD”和每种植物提取物（20毫克/千克）预处理显著增加了谷胱甘肽、过氧化氢酶和血红素加氧酶-1（与对照组相比<0.05）。我们的研究结果表明，“VARD”部分通过抗氧化活性可被视为一种有效的抗溃疡配方。
