Akhtar Muhammad Tahir, Almas Tahira, Safdar Samreen, Saadia Mubshara, Qadir Rahman, Batool Sajida, Mustaqeem Muhammad, Ali Shaukat Usman, Kanwal Fariha, Cai Rujie
Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
Institute of Chemistry, University of Sargodha, Sargodha 40100, Pakistan.
ACS Omega. 2023 Jun 5;8(24):21642-21652. doi: 10.1021/acsomega.3c01027. eCollection 2023 Jun 20.
The current study focused on the antioxidant potential, α-amylase inhibitory activity, and hypoglycemic, hypolipidemic, and histoprotective (pancreas and kidney) effects of polyherbal emulsion on the alloxan-induced diabetic rats. Polyherbal formulations were prepared from extracts and oils of (), (), and (). Out of nine stable formulations, one formulation named F6-SMONSECCE was found to be the best after its evaluation using antioxidant and in vitro α-amylase inhibition assay. The prepared herbal formulations showed significant ( < 0.05) antioxidant activity in terms of radical scavenging as 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric-reducing antioxidant power (FRAP) assays and also revealed the presence of a significant amount of total phenolic and flavonoid contents. "F6- SMONSECCE" (prepared with composition; oil (SMO) + extract (NSE) + extract CCE) was selected for an in vivo trial to ascertain its antidiabetic potential. The treatment dose was determined by using an acute toxicity trial on rats. Administration of alloxan (150 mg/kg b.w., i.p.) significantly ( < 0.05) augmented the blood glucose levels and lipid contents as total cholesterol (TC), triglycerides (TG), low-density lipoproteins (LDL-c), and very-low-density lipoproteins (VLDL-c). However, the levels of insulin and high-density lipoproteins (HDL-c) were found to be decreased, and the histopathological alterations were also found in the pancreas and kidney. The administration of the polyherbal formulation (F6-SMONSECCE) significantly attenuated the blood glucose levels (22.94%), TC (29.10%), TG (38.15%), LDL-c (27.58%), and VLDL-c (71.52%), whereas on the other side, the insulin (-149.15%) and HDL-c levels (-22.22%) were significantly increased. A significant histopathological normalization was observed in the pancreas and kidney tissues of the F6-SMONSECCE-treated rats. The current findings proposed that the prepared polyherbal formulation "F6-SMONSECCE" exhibited significant antioxidant, antilipidemic, and hypoglycemic potential and hence might be suggested as a remedy against diabetes or as a coadjuvant to synthetic medicines to maintain normal physiology.
本研究聚焦于多草药乳剂对四氧嘧啶诱导的糖尿病大鼠的抗氧化潜力、α-淀粉酶抑制活性以及降血糖、降血脂和组织保护（胰腺和肾脏）作用。多草药配方由（）、（）和（）的提取物及油类制备而成。在九种稳定配方中，经抗氧化和体外α-淀粉酶抑制试验评估后，发现一种名为F6-SMONSECCE的配方效果最佳。所制备的草药配方在2,2-二苯基-1-苦基肼（DPPH）自由基清除和铁还原抗氧化能力（FRAP）试验中表现出显著（<0.05）的抗氧化活性，并且还显示含有大量的总酚和黄酮含量。选择“F6-SMONSECCE”（由以下成分制备；油（SMO）+提取物（NSE）+提取物CCE）进行体内试验，以确定其抗糖尿病潜力。治疗剂量通过对大鼠进行急性毒性试验来确定。注射四氧嘧啶（150mg/kg体重，腹腔注射）显著（<0.05）提高了血糖水平和脂质含量，如总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL-c）和极低密度脂蛋白（VLDL-c）。然而，胰岛素和高密度脂蛋白（HDL-c）水平降低，并且在胰腺和肾脏中也发现了组织病理学改变。给予多草药配方（F6-SMONSECCE）显著降低了血糖水平（22.94%）、TC（29.10%）、TG（38.15%）、LDL-c（27.58%）和VLDL-c（71.52%），而另一方面，胰岛素（-149.15%）和HDL-c水平（-22.22%）显著升高。在F6-SMONSECCE治疗的大鼠的胰腺和肾脏组织中观察到显著的组织病理学正常化。目前的研究结果表明，所制备的多草药配方“F6-SMONSECCE”具有显著的抗氧化、抗血脂和降血糖潜力，因此可能被建议作为糖尿病的治疗药物或作为合成药物的辅助药物以维持正常生理功能。
