Department of Endodontics, Jinan Stomatological Hospital, Jinan, Shandong, People's Republic of China.
Department of Oral Medicine, Liaocheng Peoples Hospital, Liaocheng, Shandong, People's Republic of China.
J Mol Histol. 2020 Dec;51(6):649-658. doi: 10.1007/s10735-020-09911-7. Epub 2020 Sep 29.
Whether external root resorption is associated with hypoxia in the periodontal ligaments of teeth with severe periodontitis remains unclear. Hypoxia inducible factor-1α (HIF-1α) expression and external resorption sites in the periodontal ligaments of these teeth were observed to elaborate upon the relationship between hypoxia and external root resorption in severe periodontitis. Histological analysis was performed to observe external root resorption. The expressions of HIF-1α and Nuclear factor-activated T cells c1 (NFATc1) in the periodontal ligaments were detected by immunofluorescence, western blotting and real-time PCR. Bone marrow macrophages (BMMs) were stimulated by Porphyromonas gingivalis lipopolysaccharide (Pg.LPS) and cultured under hypoxia in vitro. High levels of HIF-1α and NFATc1 were detected in severe periodontitis. HIF-1α positive-cells were observed in the external resorption sites. Hypoxia promoted Pg.LPS-stimulated osteoclastogenesis of BMMs and bone resorption by the NFATc1 pathway. Increased HIF-1α in severe periodontitis are associated with external root resorption by the NFATc1 pathway.
严重牙周炎牙齿牙周韧带中的缺氧是否与外部牙根吸收有关尚不清楚。观察这些牙齿牙周韧带中缺氧诱导因子-1α（HIF-1α）的表达和外部吸收部位，以阐述严重牙周炎中缺氧与外部牙根吸收之间的关系。进行组织学分析以观察外部牙根吸收。通过免疫荧光、Western blot 和实时 PCR 检测牙周韧带中 HIF-1α 和核因子激活 T 细胞 c1（NFATc1）的表达。体外缺氧条件下用牙龈卟啉单胞菌脂多糖（Pg.LPS）刺激骨髓巨噬细胞（BMM）并培养。在严重牙周炎中检测到高水平的 HIF-1α 和 NFATc1。在外吸收部位观察到 HIF-1α 阳性细胞。缺氧促进 Pg.LPS 刺激的 BMM 破骨细胞形成和 NFATc1 途径的骨吸收。严重牙周炎中 HIF-1α 的增加与 NFATc1 途径的外部牙根吸收有关。
