Liu Zhirong, Liang Xi, Liu Huanhuan, Wang Zhuo, Jiang Tao, Cheng Yuanyuan, Wu Mengqi, Xiang Deli, Li Zhou, Wang Zhong Lin, Li Linlin
Chinese Academy of Sciences (CAS) Center for Excellence in Nanoscience, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, P.R. China.
School of Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100049, P.R. China.
ACS Nano. 2020 Nov 24;14(11):15458-15467. doi: 10.1021/acsnano.0c06100. Epub 2020 Oct 27.
Electroporation is an effective approach for drug and gene delivery, but it is still limited by its low-throughput and severe cell damage. Herein, with a self-powered triboelectric nanogenerator as the power source, we demonstrated a high-throughput electroporation system based on the design of biocompatible and flexible polypyrrole microfoam as the electrode within the flow channel. In particular, to lower the imposed voltage, one-dimensional (1D) Ag nanowires were modified on the microfoam electrode to build up a locally enhanced electric field and reduce cell damage. The self-powered electroporation system realized a successful delivery of small and large biomolecules into different cell lines with efficiency up to 86% and cell viability over 88%. The handle throughput achieved as high as 10 cells min on continuously flowed cells. The high-throughput and self-powered electroporation system is expected to have potential applications in the fields of high-throughput drug and gene delivery for isolated cells.
电穿孔是一种用于药物和基因递送的有效方法，但它仍然受到低通量和严重细胞损伤的限制。在此，我们以自供电摩擦纳米发电机作为电源，基于在流动通道内设计生物相容性和柔性聚吡咯微泡沫作为电极，展示了一种高通量电穿孔系统。特别地，为了降低施加电压，在微泡沫电极上修饰了一维（1D）银纳米线，以建立局部增强电场并减少细胞损伤。该自供电电穿孔系统成功地将小分子和大分子生物分子递送至不同细胞系，效率高达86%，细胞活力超过88%。在连续流动的细胞上实现的处理通量高达每分钟10个细胞。这种高通量和自供电电穿孔系统有望在分离细胞的高通量药物和基因递送领域具有潜在应用。
