Häppölä O, Soinila S, Päivärinta H, Panula P
Neuroscience. 1987 Apr;21(1):283-95. doi: 10.1016/0306-4522(87)90339-3.
[Met5]enkephalin-Arg6-Phe7-(MEAP-) and [Met5]enkephalin-Arg6-Gly7-Leu8-(MEAGL-) immunoreactivity was studied by indirect immunohistochemistry in the superior cervical ganglion of the rat with specific antisera produced in rabbits against the corresponding synthetic opioid peptides. Several MEAP- and a few MEAGL-immunoreactive principal nerve cells were observed in the ganglion, while the small intensely fluorescent cells appeared as non-reactive. The superior cervical ganglion also contained dense networks of MEAP- and MEAGL-immunoreactive nerve fibres, which often formed basket-like structures around the principal nerve cells and small intensely fluorescent cells. After ligation of the preganglionic nerve trunk with simultaneous transection of the main postganglionic trunks, a distinct accumulation of both MEAP- and MEAGL-immunoreactivity was observed on both sides of the ligature. Ligation of the preganglionic nerve trunk caused a marked decrease in the number of both MEAP- and MEAGL-immunoreactive nerve fibres in the ganglion. Ligation of the main postganglionic nerve trunks with simultaneous preganglionic nerve division resulted in accumulation of MEAP- and MEAGL-immunoreactive material on the ganglionic side of the ligature in both the external and internal carotid nerve. After division of both the pre- and postganglionic nerve trunks, some immunoreactive nerve fibres and principal nerve cells were still observed in the ganglion. A few immunoreactive neurons and nerve fibres were also observed in the ganglion stellatum. A large number of MEAP- and MEAGL-immunoreactive nerve fibres was detected in the spinal cord at the levels C6-Th6. A few neurons in the intermediolateral cell column of the spinal cord at levels C8-Th1 showed MEAP- but not MEAGL-immunoreactivity. The cultured superior cervical ganglion contained a few MEAP-immunoreactive neurons, and the fibre outgrowth showed immunoreactivity both to MEAP and MEAGL. In electron microscopy, MEAGL-immunoreactivity in the superior cervical ganglion was localized in nerve fibres containing neurotubules and in principal nerve cells. The present results demonstrate that the rat superior cervical ganglion contains both extrinsic and intrinsic MEAP- and MEAGL-immunoreactive nerve fibres. Most of these fibres are of preganglionic origin. Both the principal nerve and small intensely fluorescent cells are often surrounded by MEAP- or MEAGL-immunoreactive nerve fibres and may receive innervation by these fibres. Several ganglionic neurons projecting to the sympathetic target tissues show MEAP- and/or MEAGL-immunoreactivity.(ABSTRACT TRUNCATED AT 250 WORDS)
用兔抗相应合成阿片肽产生的特异性抗血清，通过间接免疫组织化学法研究了大鼠颈上神经节中[Met5]脑啡肽-Arg6-Phe7-(MEAP-)和[Met5]脑啡肽-Arg6-Gly7-Leu8-(MEAGL-)的免疫反应性。在神经节中观察到几个MEAP-免疫反应性和少数MEAGL-免疫反应性的主要神经细胞，而小而强荧光细胞显示无反应性。颈上神经节还含有密集的MEAP-和MEAGL-免疫反应性神经纤维网络，这些纤维常常在主要神经细胞和小而强荧光细胞周围形成篮状结构。在结扎节前神经干并同时横断主要节后神经干后，在结扎线两侧均观察到MEAP-和MEAGL-免疫反应性物质的明显积聚。结扎节前神经干导致神经节中MEAP-和MEAGL-免疫反应性神经纤维数量显著减少。结扎主要节后神经干并同时切断节前神经，导致颈外和颈内神经结扎线神经节侧MEAP-和MEAGL-免疫反应性物质积聚。在切断节前和节后神经干后，在神经节中仍观察到一些免疫反应性神经纤维和主要神经细胞。在星状神经节中也观察到少数免疫反应性神经元和神经纤维。在C6-Th6水平的脊髓中检测到大量MEAP-和MEAGL-免疫反应性神经纤维。在C8-Th1水平脊髓中间外侧细胞柱中的少数神经元显示MEAP-免疫反应性，但无MEAGL-免疫反应性。培养的颈上神经节含有少数MEAP-免疫反应性神经元，其纤维生长对MEAP和MEAGL均显示免疫反应性。在电子显微镜下，颈上神经节中的MEAGL-免疫反应性定位于含有神经微管的神经纤维和主要神经细胞中。目前的结果表明，大鼠颈上神经节含有外在和内在的MEAP-和MEAGL-免疫反应性神经纤维。这些纤维大多数起源于节前。主要神经细胞和小而强荧光细胞常常被MEAP-或MEAGL-免疫反应性神经纤维包围，并可能接受这些纤维的支配。投射到交感靶组织的几个神经节神经元显示MEAP-和/或MEAGL-免疫反应性。（摘要截短至250字）
