Häppölä O, Lakomy M, Yanaihara N
Department of Anatomy, University of Helsinki, Finland.
Histochemistry. 1990;93(5):479-84. doi: 10.1007/BF00266404.
Indirect immunofluorescence method was used to study the localization and distribution of the proenkephalin A-derived octapeptide, Met5-enkephalin-Arg6-Gly7-Leu8 (MEAGL), in the paracervical ganglion and in the female genital organs of the pig. In the paracervical ganglion, a subpopulation of principal neurons and nerve fibers contained MEAGL immunoreactivity. In the vagina, numerous MEAGL-immunoreactive nerve fibers were localized in the muscular membrane, under the serous membrane and in the submucous layer. The uterine cervix contained a great number of immunoreactive nerve fibers in muscular membrane and in submucous and subserous layers. The pattern of distribution of MEAGL-immunoreactive nerve fibers in the uterine horns was similar to that of cervix, but their number in the uterine horns was lower. MEAGL-immunoreactive fibers were also observed through different oviductal layers. In the ovary a low number of immunoreactive fibers were seen in the medullary and cortical parts of the organ. The results of this study indicate that the female genital organs, particularly the uterus and vagina, of the pig receive dense innervation by nerve fibers containing the proenkephalin A-derived octapeptide MEAGL. The presence of MEAGL in principal neurons and fibers of the paracervical ganglion suggests that a large proportion of them originate from neurons of the paracervical ganglion.
采用间接免疫荧光法研究了猪脑啡肽原A衍生的八肽（Met5-脑啡肽-Arg6-Gly7-Leu8，MEAGL）在子宫旁神经节和雌性生殖器官中的定位和分布。在子宫旁神经节中，一群主要神经元和神经纤维含有MEAGL免疫反应性。在阴道中，大量MEAGL免疫反应性神经纤维定位于肌膜、浆膜下和黏膜下层。子宫颈在肌膜、黏膜下层和浆膜下层含有大量免疫反应性神经纤维。子宫角中MEAGL免疫反应性神经纤维的分布模式与子宫颈相似，但子宫角中的数量较少。在输卵管的不同层也观察到了MEAGL免疫反应性纤维。在卵巢中，在器官的髓质和皮质部分可见少量免疫反应性纤维。本研究结果表明，猪的雌性生殖器官，尤其是子宫和阴道，接受含有脑啡肽原A衍生八肽MEAGL的神经纤维的密集支配。子宫旁神经节主要神经元和纤维中MEAGL的存在表明，其中很大一部分起源于子宫旁神经节的神经元。
