Soinila J, Salo A, Uusitalo H, Soinila S, Yanaihara N, Häppölä O
Department of Anatomy, University of Helsinki, Finland.
Cell Tissue Res. 1991 Apr;264(1):15-22. doi: 10.1007/BF00305718.
The localization of the proenkephalin A-derived octapeptide, Met5-enkephalin-Arg6-Gly7-Leu8 (MEAGL), was studied in the major salivary glands of Sprague-Dawley and Wistar rats with the indirect immunofluorescence method. MEAGL-immunoreactive nerve fibers were found around the acini, along intra- and interlobular salivary ducts and in close contact with blood vessels. In the parotid and submandibular glands tyrosine hydroxylase (TH) immunoreactivity was observed in nerve fibers around the acini, in association with intra- and interlobular salivary ducts and around blood vessels, while in the sublingual gland TH-immunoreactive nerve fibers were only seen around blood vessels. Parasympathetic neurons in submandibular ganglia contained MEAGL immunoreactivity. Moderate TH immunoreactivity was seen in some neurons of the submandibular ganglia. A subpopulation of sympathetic principal neurons in the superior cervical ganglion were immunoreactive for both MEAGL and TH. In the trigeminal ganglion, no MEAGL-immunoreactive sensory neurons or nerve fibers were observed. Superior cervical ganglionectomies resulted in a complete disappearance of TH-immunoreactive nerve fibers, while MEAGL-immunoreactive nerve fibers were still present in the glands. The presence of MEAGL immunoreactivity in neurons of both sympathetic superior cervical ganglia and parasympathetic submandibular ganglia and the results of superior cervical ganglionectomies suggest, that MEAGL-immunoreactive nerve fibers in the major salivary glands of the rat have both sympathetic and parasympathetic origin.
采用间接免疫荧光法研究了源自前脑啡肽A的八肽，即甲硫氨酸脑啡肽-精氨酸-甘氨酸-亮氨酸（MEAGL）在Sprague-Dawley大鼠和Wistar大鼠主要唾液腺中的定位。在腺泡周围、小叶内和小叶间唾液导管周围以及与血管紧密接触处发现了MEAGL免疫反应性神经纤维。在腮腺和下颌下腺中，在腺泡周围、小叶内和小叶间唾液导管周围以及血管周围的神经纤维中观察到酪氨酸羟化酶（TH）免疫反应性，而在舌下腺中，仅在血管周围可见TH免疫反应性神经纤维。下颌下神经节中的副交感神经元含有MEAGL免疫反应性。在一些下颌下神经节神经元中可见中度TH免疫反应性。颈上神经节中的一部分交感节前神经元对MEAGL和TH均呈免疫反应性。在三叉神经节中，未观察到MEAGL免疫反应性感觉神经元或神经纤维。切除颈上神经节后，TH免疫反应性神经纤维完全消失，而MEAGL免疫反应性神经纤维仍存在于腺体中。颈上神经节和下颌下神经节神经元中MEAGL免疫反应性的存在以及颈上神经节切除的结果表明，大鼠主要唾液腺中MEAGL免疫反应性神经纤维具有交感和副交感双重起源。
