Aryal Babita, Niraula Purushottam, Khadayat Karan, Adhikari Bikash, Khatri Chhetri Dadhiram, Sapkota Basanta Kumar, Bhattarai Bibek Raj, Aryal Niraj, Parajuli Niranjan
Biological Chemistry Lab, Central Department of Chemistry, Tribhuvan University, Kirtipur, Nepal.
Pharmaceutical Institute, Department of Pharmaceutical Biology, University of Tübingen, Tübingen, Germany.
Evid Based Complement Alternat Med. 2021 May 6;2021:5510099. doi: 10.1155/2021/5510099. eCollection 2021.
Natural products have been the center of attraction ever since they were discovered. Among them, plant-based natural products were popular as analgesics, anti-inflammatory, antidiabetic, and cosmetics and possess widespread biotechnological applications. The use of plant products as cosmetics and therapeutics is deep-rooted in Nepalese society. Although there are few ethnobotanical studies conducted, extensive research of these valuable medicinal plants has not been a priority due to the limitation of technology and infrastructure. Here, we selected 4 traditionally used medicinal plants to examine their bioactive properties and their enzyme inhibition potential. -Glucosidase and -amylase inhibitory activities were investigated using an model followed up by antioxidant and antimicrobial activities. The present study shows that ethyl acetate fraction of (IC 9.1 ± 0.3 g/mL) and water fraction (IC 9.0 ± 0.6 g/mL) exhibit strong -glucosidase inhibition. Likewise, the highest amylase inhibition was shown by crude extracts of (IC 29.2 ± 1.2 g/mL) and ethyl acetate fractions of (IC 69.3 ± 1.1 g/mL), and the highest radical scavenging activity was shown by with an IC 67.4 ± 0.6 g/mL. Furthermore, to identify the metabolites within the fractions, we employed high-resolution mass spectrometry (LC-HRMS) and annotated 17 known metabolites which justify our assumption on activity. Of 4 medicinal plants examined, ethyl acetate fraction of , ethyl acetate fraction of , and water or ethyl acetate fraction of extracts illustrated the best activities. With our study, we set up a foundation that provides authentic evidence to the community for use of these traditional plants. The annotated metabolites in this study support earlier experimental evidence towards the inhibition of enzymes. Further study is necessary to explore the clinical efficacy of these secondary molecules, which might be alternatives for the treatment of diabetes and pathogens.
自从天然产物被发现以来，它们一直是人们关注的焦点。其中，植物源天然产物作为镇痛药、抗炎药、抗糖尿病药和化妆品广受欢迎，并具有广泛的生物技术应用。在尼泊尔社会，将植物产品用作化妆品和治疗剂有着深厚的根基。尽管进行的民族植物学研究较少，但由于技术和基础设施的限制，对这些珍贵药用植物的广泛研究并非优先事项。在此，我们选择了4种传统使用的药用植物，以研究它们的生物活性特性及其酶抑制潜力。使用体外模型研究了α-葡萄糖苷酶和α-淀粉酶抑制活性，随后研究了抗氧化和抗菌活性。本研究表明，[植物名称1]的乙酸乙酯馏分（IC50 9.1±0.3μg/mL）和水馏分（IC50 9.0±0.6μg/mL）表现出强烈的α-葡萄糖苷酶抑制作用。同样，[植物名称2]的粗提物（IC50 29.2±1.2μg/mL）和[植物名称3]的乙酸乙酯馏分（IC50 69.3±1.1μg/mL）表现出最高的淀粉酶抑制作用，而[植物名称4]的自由基清除活性最高，IC50为67.4±0.6μg/mL。此外，为了鉴定馏分中的代谢物，我们采用了高分辨率质谱（LC-HRMS），并注释了17种已知代谢物，这证实了我们对活性的假设。在所研究的4种药用植物中，[植物名称1]的乙酸乙酯馏分、[植物名称3]的乙酸乙酯馏分以及[植物名称4]提取物的水或乙酸乙酯馏分表现出最佳活性。通过我们的研究，我们建立了一个基础，为社区使用这些传统植物提供了可靠的证据。本研究中注释的代谢物支持了早期关于酶抑制的实验证据。有必要进一步研究这些次生分子的临床疗效，它们可能是治疗糖尿病和病原体的替代物。
