壳聚糖纳米粒子作为可食用的表面涂层剂来保鲜辣椒（Capsicum annuum L. var. grossum (L.) Sendt）。
Chitosan nanoparticles as edible surface coating agent to preserve the fresh-cut bell pepper (Capsicum annuum L. var. grossum (L.) Sendt).
机构信息
Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, South Korea.
Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, South Korea.
出版信息
Int J Biol Macromol. 2020 Dec 15;165(Pt A):948-957. doi: 10.1016/j.ijbiomac.2020.09.176. Epub 2020 Sep 28.
This work synthesized chitosan nanoparticles (CSNPs) by ionic gelation method using sodium tripolyphosphate pentabasic (STPP) and applied as nano-coating agent to extend the shelf life of fresh cut bell pepper (FCP) by preventing the microbial contaminations. The CSNPs were spherical shaped and 22.55 ± 1.69 d.nm sized with ζ-potential of 45.10 ± 1.42 mV confirmed by UHR-SEM, FE-TEM, and ζ-potential size analyses. The crystallinity and functional group changes were determined by XRD and FT-IR analyses respectivelly. The antioxidant activity of CSNPs was determined in terms of DPPH and ABTS scavenging activities. The 1% and 3% of CSNPs were found to be minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) respectively against all tested bacteria. The MBC of CSNPs was exhibited the promising anti-biofilm activity. Therefore, the 3% of CSNPs was used to prepare the chitosan nano-coating (CSNC), and applied on surface of FCP to prevent the microbial contaminations such as fungi, bacteria including Listeria monocytogenes and Salmonella enterica. The experimental results showed that the application of CSNC was maintained the FCP for 12 days at 5 °C without loss of weight, and sensory quality.
本工作采用离子凝胶法合成壳聚糖纳米粒子（CSNPs），使用三聚磷酸五钠（STPP）作为交联剂，将其作为纳米涂层剂应用于鲜切甜椒（FCP），以防止微生物污染，从而延长其货架期。CSNPs 呈球形，粒径为 22.55±1.69 d.nm，ζ-电位为 45.10±1.42 mV，通过 UHR-SEM、FE-TEM 和 ζ-电位大小分析得到证实。通过 XRD 和 FT-IR 分析分别确定了结晶度和官能团的变化。CSNPs 的抗氧化活性通过 DPPH 和 ABTS 清除活性来测定。结果发现，1%和 3%的 CSNPs 分别是所有测试细菌的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。CSNPs 的 MBC 表现出有希望的抗生物膜活性。因此，将 3%的 CSNPs 用于制备壳聚糖纳米涂层（CSNC），并将其应用于 FCP 的表面，以防止真菌、细菌（包括单核细胞增生李斯特菌和肠炎沙门氏菌）等微生物的污染。实验结果表明，CSNC 的应用可使 FCP 在 5°C 下保持 12 天而不损失重量和感官质量。
Zotero 插件