大肠杆菌单链DNA结合蛋白与mRNA的特异性协同结合
Specific and cooperative binding of E. coli single-stranded DNA binding protein to mRNA.
作者信息
Shimamoto N, Ikushima N, Utiyama H, Tachibana H, Horie K
出版信息
Nucleic Acids Res. 1987 Jul 10;15(13):5241-50. doi: 10.1093/nar/15.13.5241.
Abstract
Fluorometric titration of E. coli single-stranded DNA binding protein with various RNAs showed that the protein specifically and cooperatively binds to its own mRNA. The binding inhibited in vitro expression of ssb and bla but not nusA. This inhibition takes place at a physiological concentration of SSB. The function of the protein in gene regulation is discussed.
摘要
用各种RNA对大肠杆菌单链DNA结合蛋白进行荧光滴定表明，该蛋白能特异性且协同地结合其自身的mRNA。这种结合抑制了ssb和bla的体外表达，但不抑制nusA的表达。这种抑制在生理浓度的SSB下发生。文中讨论了该蛋白在基因调控中的功能。
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