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大肠杆菌单链DNA结合蛋白与mRNA的特异性协同结合

Specific and cooperative binding of E. coli single-stranded DNA binding protein to mRNA.

作者信息

Shimamoto N, Ikushima N, Utiyama H, Tachibana H, Horie K

出版信息

Nucleic Acids Res. 1987 Jul 10;15(13):5241-50. doi: 10.1093/nar/15.13.5241.

DOI:10.1093/nar/15.13.5241
PMID:3299265
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC305958/
Abstract

Fluorometric titration of E. coli single-stranded DNA binding protein with various RNAs showed that the protein specifically and cooperatively binds to its own mRNA. The binding inhibited in vitro expression of ssb and bla but not nusA. This inhibition takes place at a physiological concentration of SSB. The function of the protein in gene regulation is discussed.

摘要

用各种RNA对大肠杆菌单链DNA结合蛋白进行荧光滴定表明，该蛋白能特异性且协同地结合其自身的mRNA。这种结合抑制了ssb和bla的体外表达，但不抑制nusA的表达。这种抑制在生理浓度的SSB下发生。文中讨论了该蛋白在基因调控中的功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b0/305958/3a379d6f9f53/nar00257-0256-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b0/305958/a43082e0ecac/nar00257-0255-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b0/305958/3a379d6f9f53/nar00257-0256-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b0/305958/a43082e0ecac/nar00257-0255-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50b0/305958/3a379d6f9f53/nar00257-0256-a.jpg

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本文引用的文献

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Mechanism and role of cooperative binding of bacteriophage fd gene 5 protein to single-stranded deoxyribonucleic acid.噬菌体fd基因5蛋白与单链脱氧核糖核酸协同结合的机制及作用
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A profusion of controls.大量的控制措施。
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RNA-DNA hybridization promoted by E. coli RecA protein.由大肠杆菌RecA蛋白促进的RNA-DNA杂交。
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Escherichia coli single-strand binding protein stabilizes specific denatured sites in superhelical DNA.大肠杆菌单链结合蛋白可稳定超螺旋DNA中的特定解链位点。
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Limited proteolysis studies on the Escherichia coli single-stranded DNA binding protein. Evidence for a functionally homologous domain in both the Escherichia coli and T4 DNA binding proteins.大肠杆菌单链DNA结合蛋白的有限蛋白酶解研究。大肠杆菌和T4 DNA结合蛋白中功能同源结构域的证据。
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