大肠杆菌中单链DNA结合蛋白的扩增
Amplification of single-strand DNA binding protein in Escherichia coli.
作者信息
Chase J W, Whittier R F, Auerbach J, Sancar A, Rupp W D
出版信息
Nucleic Acids Res. 1980 Jul 25;8(14):3215-27. doi: 10.1093/nar/8.14.3215.
Abstract
An E. coli strain containing a recombinant plasmid carrying the E. coli ssbA+ gene has been shown to produce 12 to 15 fold increased amounts of single-strand DNA binding-protein relative to wild-type strains. In addition, a gamma transducing phage carrying the E. coli uvrA+ gene has been shown to also carry the ssbA+ gene and to be capable of producing increased amounts of binding protein.
摘要
已证明，含有携带大肠杆菌ssbA⁺基因的重组质粒的大肠杆菌菌株，相对于野生型菌株，其单链DNA结合蛋白的产量增加了12至15倍。此外，已证明携带大肠杆菌uvrA⁺基因的γ转导噬菌体也携带ssbA⁺基因，并且能够产生更多的结合蛋白。
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