Cho Yong Sang, Kim Hee-Kyeong, Ghim Min-Soo, Hong Myoung Wha, Kim Young Yul, Cho Young-Sam
Medical IT Convergence Research Section, Daegu-Gyeongbuk Research Center, Electronics and Telecommunications Research Institute (ETRI), 1, Techno Sunhwan-ro 10-gil, Dalseong-gun, Daegu 42994, Korea.
Department of Mechanical Engineering, College of Engineering, Wonkwang University, 460 Iksandae-ro, Iksan, Jeonbuk 54538, Korea.
Polymers (Basel). 2020 Sep 25;12(10):2193. doi: 10.3390/polym12102193.
Among 3D-printed composite scaffolds for bone tissue engineering, researchers have been attracted to the use of zinc ions to improve the scaffold's anti-bacterial activity and prevent surgical site infection. In this study, we assumed that the concentration of zinc ions released from the scaffold will be correlated with the thickness of the zinc oxide coating on 3D-printed scaffolds. We investigated the adequate thickness of zinc oxide coating by comparing different scaffolds' characteristics, antibacterial activity, and in vitro cell response. The scaffolds' compressive modulus decreased as the zinc oxide coating thickness increased (10, 100 and 200 nm). However, the compressive modulus of scaffolds in this study were superior to those of other reported scaffolds because our scaffolds had a kagome structure and were made of composite material. In regard to the antibacterial activity and in vitro cell response, the in vitro cell proliferation on scaffolds with a zinc oxide coating was higher than that of the control scaffold. Moreover, the antibacterial activity of scaffolds with 100 or 200 nm-thick zinc oxide coating on was superior to that of other scaffolds. Therefore, we concluded that the scaffold with a 100 nm-thick zinc oxide coating was the most appropriate scaffold to use as a bone-regenerating scaffold, given its mechanical property, its antibacterial activity, and its in vitro cell proliferation.
在用于骨组织工程的3D打印复合支架中，研究人员一直热衷于使用锌离子来提高支架的抗菌活性并预防手术部位感染。在本研究中，我们假设从支架释放的锌离子浓度将与3D打印支架上氧化锌涂层的厚度相关。我们通过比较不同支架的特性、抗菌活性和体外细胞反应来研究氧化锌涂层的合适厚度。随着氧化锌涂层厚度增加（10、100和200纳米），支架的压缩模量降低。然而，本研究中支架的压缩模量优于其他报道的支架，因为我们的支架具有 kagome 结构且由复合材料制成。关于抗菌活性和体外细胞反应，具有氧化锌涂层的支架上的体外细胞增殖高于对照支架。此外，具有100或200纳米厚氧化锌涂层的支架的抗菌活性优于其他支架。因此，考虑到其机械性能、抗菌活性和体外细胞增殖，我们得出结论，具有100纳米厚氧化锌涂层的支架是用作骨再生支架的最合适支架。
