Laboratory of Wildlife Epidemic Diseases, School of Life Sciences, East China Normal University, Shanghai 200063, China.
Pudong District Forestry Station of Shanghai, Shanghai 200120, China.
Viruses. 2020 Sep 25;12(10):1085. doi: 10.3390/v12101085.
H10, H11 and H12 (H10-H12) subtypes of the avian influenza virus (AIV) are associated with waterfowl. Although these subtypes of AIV are infrequently detected in nature, they can undergo reassortment with other AIV subtypes. Few H10-H12 subtypes of AIV have been isolated from wild birds in China. In this study, 12 AIV isolates of H10-H12 subtypes were identified via routine surveillance of wild birds in Shanghai, China from 2016 to 2019, including two H10, three H11 and seven H12 isolates. Sequence and phylogenetic analyses revealed that the genomic segments of the 12 isolates are highly diverse. These 12 isolates are closely related to those in the Eurasian lineage and share a high degree of sequence identity with those from wild birds and domestic ducks in countries in the East Asian-Australasian Flyway, including Japan, Korea, Bangladesh, Vietnam and China. However, parts of the genomic segments of two H12N2 isolates (NH112319-H12N2 and NH101807-H12N2) belong to the North American lineage, suggesting intercontinental reassortment among H12 AIVs in Eurasia and North American. To better understand the ecological and phylodynamic features of H10-H12 subtypes in wild birds, a large-scale surveillance of AIVs in wild birds is warranted.
H10、H11 和 H12（H10-H12）亚型的禽流感病毒（AIV）与水禽有关。尽管这些 AIV 亚型在自然界中很少被检测到，但它们可以与其他 AIV 亚型发生重配。在中国，从野生鸟类中分离到的 H10-H12 亚型 AIV 很少。在这项研究中，2016 年至 2019 年期间，通过对中国上海野生鸟类的常规监测，鉴定出了 12 株 H10-H12 亚型的 AIV，包括 2 株 H10、3 株 H11 和 7 株 H12 分离株。序列和系统发育分析表明，这 12 个分离株的基因组片段高度多样化。这 12 个分离株与欧亚谱系密切相关，与东亚-澳大拉西亚飞行路线国家（包括日本、韩国、孟加拉国、越南和中国）的野生鸟类和家养鸭中的病毒具有高度的序列同一性。然而，两个 H12N2 分离株（NH112319-H12N2 和 NH101807-H12N2）的部分基因组片段属于北美谱系，表明欧亚大陆和北美 H12 AIV 之间存在跨大陆重配。为了更好地了解野生鸟类中 H10-H12 亚型的生态和系统发育特征，有必要对野生鸟类中的 AIV 进行大规模监测。
