Shanghai Public Health Clinical Center & Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 201508, China.
Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, Korea.
Int J Mol Sci. 2020 Sep 26;21(19):7114. doi: 10.3390/ijms21197114.
(1) Background: Aluminum oxide (AlO) ceramic is one of the materials used for artificial joints, and it has been known that their fine particles (FPs) are provided by the wear of the ceramic. AlO FPs have been shown to induce macrophage activation in vitro; however, the inflammatory effect in vivo has not been studied. (2) Methods: We examined the in vivo effect of AlO FPs on the innate and adaptive immune cells in the mice. (3) Results: AlO FPs promoted the activation of spleen macrophages; however, conventional dendritic cells (cDCs), plasmacytoid DCs (pDCs), and natural killer (NK) cells were not activated. In addition, increases in the CD4 and CD8 T cells was induced in the spleens of the mice treated with AlO FPs, which differentiated into interferon-gamma (IFN-γ)-producing helper T1 (Th1) and cytotoxic T1 (Tc1) cells. Finally, the injection of AlO FPs exacerbated dextran sulfate sodium (DSS)-induced inflammation in the colon, mediated by activated and increased number of CD4 and CD8 T cells. (4) Conclusions: These data demonstrate that FPs of AlO ceramic may contribute to the exacerbation of inflammatory diseases in the patients.
(1) 背景：氧化铝（AlO）陶瓷是一种用于人工关节的材料，已知其细颗粒（FPs）是由陶瓷磨损产生的。体外研究表明，AlO FPs 可诱导巨噬细胞活化；然而，其在体内的炎症效应尚未得到研究。(2) 方法：我们研究了 AlO FPs 对小鼠固有和适应性免疫细胞的体内影响。(3) 结果：AlO FPs 促进了脾脏巨噬细胞的活化；然而，常规树突状细胞（cDCs）、浆细胞样树突状细胞（pDCs）和自然杀伤（NK）细胞未被激活。此外，用 AlO FPs 处理的小鼠脾脏中 CD4 和 CD8 T 细胞增加，分化为产生干扰素-γ（IFN-γ）的辅助性 T1（Th1）和细胞毒性 T1（Tc1）细胞。最后，AlO FPs 的注射加剧了葡聚糖硫酸钠（DSS）诱导的结肠炎症，这是由活化和数量增加的 CD4 和 CD8 T 细胞介导的。(4) 结论：这些数据表明，AlO 陶瓷的 FPs 可能有助于加重患者的炎症性疾病。
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