CEACAM5 stimulates the progression of non-small-cell lung cancer by promoting cell proliferation and migration.

OBJECTIVE

To detect the expression of CEA-related cell adhesion molecule 5 (CEACAM5) in non-small-cell lung cancer (NSCLC) and explore its function in the progression and development of NSCLC.

METHODS

qRT-PCR and immunohistochemistry were performed to detect CEACAM5 expression in human NSCLC tissues and cell lines. The correlation between CEACAM5 expression and the clinicopathological features of patients with NSCLC was also investigated. MTT, colony formation, wound healing, and immunoblot assays were performed to detect the functions of CEACAM5 in NSCLC cells , and immunoblotting was used to detect the effects of CEACAM5 on p38-Smad2/3 signaling.

RESULTS

CEACAM5 expression was elevated in human NSCLC tissues and cells. We further found that CEACAM expression was correlated with clinicopathological features including T division, lymph invasion, and histological grade in patients with NSCLC. The assays confirmed that CEACAM5 depletion inhibited the proliferation and migration of NSCLC cells by activating p38-Smad2/3 signaling. We verified the involvement of CEACAM5 in the suppression of NSCLC tumor growth in mice.

CONCLUSION

CEACAM5 stimulated the progression of NSCLC by promoting cell proliferation and migration and . CEACAM5 may serve as a potential therapeutic target for the treatment of NSCLC.