Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs/ Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, Guangdong, China.
BMC Plant Biol. 2020 Sep 29;20(1):447. doi: 10.1186/s12870-020-02671-2.
To explore the molecular regulatory mechanisms of early stem and leaf development, proteomic analysis was performed on leaves and stems of F genotype alfalfa, with thin stems and small leaves, and M genotype alfalfa, with thick stems and large leaves.
Based on fold-change thresholds of > 1.20 or < 0.83 (p < 0.05), a large number of proteins were identified as being differentially enriched between the M and F genotypes: 249 downregulated and 139 upregulated in stems and 164 downregulated and 134 upregulated in leaves. The differentially enriched proteins in stems were mainly involved in amino acid biosynthesis, phenylpropanoid biosynthesis, carbon fixation, and phenylalanine metabolism. The differentially enriched proteins in leaves were mainly involved in porphyrin and chlorophyll metabolism, phenylpropanoid biosynthesis, starch and sucrose metabolism, and carbon fixation in photosynthetic organisms. Six differentially enriched proteins were mapped onto the porphyrin and chlorophyll metabolism pathway in leaves of the M genotype, including five upregulated proteins involved in chlorophyll biosynthesis and one downregulated protein involved in chlorophyll degradation. Eleven differentially enriched proteins were mapped onto the phenylpropanoid pathway in stems of the M genotype, including two upregulated proteins and nine downregulated proteins.
Enhanced chlorophyll synthesis and decreased lignin synthesis provided a reasonable explanation for the larger leaves and lower levels of stem lignification in M genotype alfalfa. This proteomic study aimed to classify the functions of differentially enriched proteins and to provide information on the molecular regulatory networks involved in stem and leaf development.
为了探索早期茎和叶发育的分子调控机制，对茎细叶小的 F 基因型苜蓿和茎粗叶大的 M 基因型苜蓿的叶片和茎进行了蛋白质组学分析。
基于倍数变化阈值>1.20 或<0.83（p<0.05），大量蛋白质被鉴定为在 M 和 F 基因型之间差异富集：在茎中 249 个下调和 139 个上调，在叶片中 164 个下调和 134 个上调。在茎中差异富集的蛋白质主要涉及氨基酸生物合成、苯丙烷生物合成、碳固定和苯丙氨酸代谢。在叶片中差异富集的蛋白质主要涉及卟啉和叶绿素代谢、苯丙烷生物合成、淀粉和蔗糖代谢以及光合生物的碳固定。在 M 基因型叶片中，有 6 个差异富集蛋白映射到卟啉和叶绿素代谢途径上，包括 5 个参与叶绿素生物合成的上调蛋白和 1 个参与叶绿素降解的下调蛋白。在 M 基因型茎中，有 11 个差异富集蛋白映射到苯丙烷途径上，包括 2 个上调蛋白和 9 个下调蛋白。
增强的叶绿素合成和减少的木质素合成为 M 基因型苜蓿具有较大的叶片和较低的茎木质化水平提供了合理的解释。这项蛋白质组学研究旨在对差异富集蛋白的功能进行分类，并为茎和叶发育涉及的分子调控网络提供信息。
