Moosavi-Nasab Marzieh, Yazdani-Dehnavi Marzieh, Mirzapour-Kouhdasht Armin
Department of Food Science and Technology School of Agriculture Shiraz University Shiraz Iran.
Seafood Processing Research Group School of Agriculture Shiraz University Shiraz Iran.
Food Sci Nutr. 2020 Aug 5;8(9):5017-5025. doi: 10.1002/fsn3.1799. eCollection 2020 Sep.
The objective of this study was to investigate the effects of the enzymatic aided acid-swelling process on gelatin obtained from fish by-products. For this purpose, gelatin was extracted by an acidic swelling procedure in the presence of protease extracted from Rainbow trout pyloric caeca. The yield of gelatin extraction and the most important physicochemical characteristics of the fish gelatin samples were investigated and compared with those of commercial bovine gelatin (CBG). The yields of gelatin from skin (ESG) either with or without crude protease from pyloric caeca (15 units/g alkaline treated) were 14.98% and 50.89%, respectively. The yields of gelatin from scales (CSG) with crude protease from pyloric caeca (15 units/g) were 49.97%. The gel strength of the CSG (259.66 g) was significantly higher than that of CBG (228.30 g) and ESG (187.75 g). Similarly, the gelling and melting points, foaming capacity and stability, and the SDS-PAGE pattern of gelatins were compared. The electrophoretic pattern confirmed the results of gel strength which was due to the narrower alpha and beta bands in fish skin and commercial bovine gelatins than that of fish scales gelatin. The results of this research showed that the production of high-quality gelatin can be achieved by the enzymatically aided acid-swelling procedure from fish scales and skin.
本研究的目的是探究酶辅助酸溶胀工艺对从鱼副产品中获得的明胶的影响。为此，在从虹鳟幽门盲囊中提取的蛋白酶存在的情况下，通过酸性溶胀法提取明胶。研究了鱼明胶样品的明胶提取率以及最重要的理化特性，并与商业牛明胶（CBG）进行了比较。有或没有来自幽门盲囊的粗蛋白酶（15单位/克碱处理）时，从鱼皮中提取明胶（ESG）的产率分别为14.98%和50.89%。使用来自幽门盲囊的粗蛋白酶（15单位/克）从鱼鳞中提取明胶（CSG）的产率为49.97%。CSG的凝胶强度（259.66克）显著高于CBG（228.30克）和ESG（187.75克）。同样，对明胶的凝胶化和熔点、发泡能力和稳定性以及SDS-PAGE图谱进行了比较。电泳图谱证实了凝胶强度的结果，这是由于鱼鳞明胶中的α和β带比鱼皮明胶和商业牛明胶中的带更窄。本研究结果表明，通过酶辅助酸溶胀工艺从鱼鳞和鱼皮中可以生产出高质量的明胶。
