Gan Yi, Liang Jie, Diao Wenjing, Zhou Xianrong, Mu Jianfei, Pang Liang, Tan Fang, Zhao Xin
Chongqing Collaborative Innovation Center for Functional Food Chongqing University of Education Chongqing China.
Chongqing Engineering Research Center of Functional Food Chongqing University of Education Chongqing China.
Food Sci Nutr. 2020 Aug 7;8(9):5128-5137. doi: 10.1002/fsn3.1814. eCollection 2020 Sep.
Constipation is a common clinical manifestation of digestive system disorders and occurs worldwide. This study investigated the ability of KSFY06 (LP-KSFY06) to promote the action of geniposide in preventing montmorillonite-induced constipation in Kunming mice, with the aim of providing a successful solution. The effects of LP-KSFY06 and geniposide on constipation were measured, and the results showed that the protective effect of geniposide on constipation was enhanced by LP-KSFY06 and that the combination resulted in increased weight, moisture content, and particle number of feces. The first black stool defecation time was decreased from 182 min to 87 min, which clearly indicates that defecating difficulty was alleviated in constipated mice. The synergic intervention of LP-KSFY06 and geniposide (LP + G) assisted in maintaining the body weight of constipated mice. The LP + G intervention significantly increased serum levels of motilin (MTL, 167.8 pg/ml), acetylcholinesterase (AChE, 45.3 pg/ml), substance P (SP, 61.0 pg/ml), vasoactive intestinal peptide (VIP, 70.5 pg/ml), endothelin-1 (ET-1, 16.1 pg/ml), and gastrin (73.0 pg/ml) and remarkably decreased somatostatin (SS, 35.2 pg/ml) when compared to those indexes in the LP-KSFY06 group and geniposide group. The LP + G treatment also significantly increased the mRNA expression of cluster of differentiation 117 (c-Kit), stem cell factor (SCF), glial cell-derived neurotrophic factor (GDNF), and remarkably downregulated the expression of inducible nitric oxide synthase (iNOS), transient receptor potential vanilloid-1 (TRPV1), and cyclooxygenase-2 (COX-2). The experimental results showed that the combination treatment has the strongest prevention effect against constipation, and LP-KSFY06 promotes the ability of geniposide to prevent constipation. Therefore, LP-KSFY06 is a potential probiotic strain with the capacity to prevent montmorillonite-induced constipation.
便秘是消化系统疾病常见的临床表现，在全球范围内都有发生。本研究探讨了KSFY06（LP-KSFY06）促进栀子苷预防蒙脱石诱导的昆明小鼠便秘的作用，旨在找到一个成功的解决方案。检测了LP-KSFY06和栀子苷对便秘的影响，结果表明，LP-KSFY06增强了栀子苷对便秘的保护作用，二者联合使粪便重量、水分含量和颗粒数增加。首次排黑便时间从182分钟降至87分钟，这清楚地表明便秘小鼠的排便困难得到缓解。LP-KSFY06和栀子苷（LP + G）的协同干预有助于维持便秘小鼠的体重。与LP-KSFY06组和栀子苷组的这些指标相比，LP + G干预显著提高了胃动素（MTL，167.8 pg/ml）、乙酰胆碱酯酶（AChE，45.3 pg/ml）、P物质（SP，61.0 pg/ml）、血管活性肠肽（VIP，70.5 pg/ml）、内皮素-1（ET-1，16.1 pg/ml）和胃泌素（73.0 pg/ml）的血清水平，并显著降低了生长抑素（SS，35.2 pg/ml）。LP + G处理还显著增加了分化簇117（c-Kit）、干细胞因子（SCF）、胶质细胞源性神经营养因子（GDNF）的mRNA表达，并显著下调了诱导型一氧化氮合酶（iNOS）、瞬时受体电位香草酸亚型1（TRPV1）和环氧化酶-2（COX-2）的表达。实验结果表明，联合治疗对便秘的预防作用最强，LP-KSFY06促进了栀子苷预防便秘的能力。因此，LP-KSFY06是一种具有预防蒙脱石诱导便秘能力的潜在益生菌菌株。
